30
For more information, or to download product instructions,
Thermo Scientific Derivatization and Visualization Reagents for HPLC
Detection Reagents
References
1. Durst, H.D.,
et al.
(1975).
Anal. Chem.
47,
1797.
2. Borch, R.F.,
et al.
(1975).
Anal. Chem.
47,
2437.
3. Grushka, E.
et al.
(1975).
J. Chromatogr.
112,
673.
4. Fitzpatrick, F.A. (1976).
Anal. Chem.
48,
499.
5. Nagels, L.,
et al.
(1980).
J. Chromatogr.
190,
411.
6. Ahmed, M.S.,
et al.
(1980).
J. Chromatogr.
192,
387.
7. Pierce Technical Bulletin: Preparation of Phenacyl and
p
-Bromophenacyl
Derivatives for HPLC.
8. Stocchi, V.,
et al.
(1985).
J. Chromatogr.
349,
77-82.
9. Chang, J.Y.,
et al.
. (1981).
Biochem. J.
199,
547-555.
10. Chang, J.Y.,
et al.
(1982).
Biochem. J.
203,
803-806.
11. Vendrell, J.,
et al.
(1986).
J. Chromatogr.
358,
401-413.
12. Chang, J.Y.,
et al.
(1984).
J. Chromatogr.
295,
193-200.
13. Lin J.K.,
et al.
(1980).
Anal. Chem.
52,
630-635.
14. Chang, J.Y.,
et al.
(1981).
FEBS Letters
132,
117-120.
15. Marfey, P. (1984).
Carlsberg Res. Comm.
49,
591-596.
16. Jones, B.N. and Gilligan, J.P. (1983).
Amer. Biotech. Lab.
12,
46-51.
17. Fiedler, H.P.,
et al.
(1986).
J. Chromatogr.
353,
201-206.
18. Fried, V.A.,
et al.
(1985).
Anal. Biochem.
146,
271-276.
19. Jones, B.N.,
et al.
(1983).
J. Chromatogr.
266,
471-482.
20. Seaver, S.S.,
et al.
(Sept./Oct. 1984).
Biotechniques
254-260.
21. Böhlen, P.,
et al.
(1979).
Anal. Biochem
.
94,
313-321.
22. Stein, W.H. and Moore, S. (1949).
Cold Spring Harbor Symp. Quant.
Bio.
14,
179.
23. Heinrickson, R.L. and Meredith, S.C. (1984).
Anal. Biochem.
137, 65-74.
24. Scholze, H. (1985).
J. Chromatogr.
350,
453-460.
25. Caudill, W.L.,
et al.
(1982).
J. Chromatogr.
227, 331.
26. Caudill, W.L.,
et al.
(1982).
Bioanalytical System’s Current Separations
4(4),
59.
27. Andrews, J.L.,
et al.
(1982).
Arch. Biochem. Biophys.
214,
386-396.
28. Aberhart, D.J.,
et al.
(1985).
Anal. Biochem.
151,
88-91.
29. Szokan, G.,
et al.
(1988).
J. Chromatogr.
444,
115-122.
Thermo Scientific Detection Reagents for HPLC
Functional Group
Description
Detection* Page
Comments
Carboxylic Acid
C
O
OH
R
p
-Bromophenacylate
C
O
CH
2
Br
Br
p-Bromophenacylate
MW 277.94
UV
31
Formulation of 1.0 mmol/ml
p
-bromophenacyl bromide
and 0.005 mmol/ml crown ether in acetonitrile;
pre-column; nanomole detection levels:
λ
max
= 260 nm
1-7
Primary Amine
N
H
H R
Dabsyl Chloride
N
N
N
S
Cl
O
O
Dabsyl Chloride
MW 323.80
Vis
41
4-N, N-dimethylaminoazobenzene-4'-sulfonyl chloride
(dabsyl chloride); pre-column; nanomole detection levels:
λ
max
= 436 nm
8-14
FDAA, Marfey’s Reagent
H
N
NH
2
O
F
N
+
–
O
O
N
+
O
–
O
FDAA
(Marfey's Reagent)
MW 272.19
UV
31, 41
1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA);
pre-column; nanomole detection levels:
λ
max
= 340 nm.
For chiral separations of amino acids.
15, 28-29
Ninhydrin
O
O
OH
OH
Ninhydrin
MW 178.14
Vis
37
Post-column; nanomole detection levels:
λ
max
= 570 nm
22
PITC
N
C
S
PITC
Edman‘s Reagent
MW 135.19
UV
40
Phenylisothiocyanate (PITC); pre-column; picomole
detection levels:
λ
max
= 254 nm
23-24
TNBSA
N
+
–
O
O
N
+
O
–
O
N
+
O
–
O
S
OH
O
O
TNBSA
MW 293.17
EC, UV
35
2,4,6-Trinitrobenzene-sulfonic acid (TNBSA); pre- or
post-column; nanomole detection levels with EC and UV,
GC - 0.85V;
λ
max
= 250 nm
25-26
Secondary Amine
NH R
′
R
Ninhydrin
(see structure above)
Vis
37
Post-column; nanomole detection levels:
λ
max
= 440 nm
22
PITC
(see structure above)
UV
40
Phenylisothiocyanate (PITC); pre-column; picomole
detection levels:
λ
max
= 254 nm
23-24
*EC = electrochemical; F = fluorescence; UV = ultraviolet; Vis = visible.
