Thermo Scientific
| Reagents, Solvents and Accessories
35
Thermo Scientific
Fluorometric Detection Reagents for Amino Acids
TNBSA
An excellent choice for spectrophotometric detection.
N
+
–
O
O
N
+
O
–
O
N
+
O
–
O
S
OH
O
O
TNBSA
MW 293.17
Trinitrobenzene sulfonic acid (TNBSA) reacts readily with
the primary amino groups of amino acids in aqueous
solution at pH8 to form yellow adducts. No colored
derivatives are formed with the secondary amino acids
proline and hydroxyproline. The colored derivatives are
monitored at 345 nm and have extinction coefficients in the
range of 1-1.5 x 10
4
.
TNBSA has been used as a hydrophilic modifying reagent
for the detection of primary amines in samples containing
amino acids, peptides or proteins. It is an excellent reagent
for rapid qualitative and quantitative estimation of these
biomolecules.
Highlights:
• Couples with primary amines, sulfhydryls and hydrazides
in aqueous solution at pH 8, without undesirable side
reactions
• Excellent for solution or solid phase analysis
• Chromogenic (
λ
max
= 335 nm)
References
1. Goodwin, J.F. and Choi, S-Y. (1970)
Clinical Chemistry.
16,
24-31.
2. Snyder, S.L. and Sobocinski, P.Z. (1975)
Anal Biochem.
64,
284-288.
3. Drozdovskaya, N.R., et al. (1982)
FEBS Lett.
150,
385
4. Takahashi, S.,
et al.
(1984)
Chem. Lett.
(Jpn).
1,
127
Ordering Information
Product # Description
Pkg. Size
✖
TS-28997
TNBSA
100 ml
(2,4,6-Trinitrobenzene sulfonic acid;
5% w/v methanol solution)
✖
Additional hazardous handling charge.
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