Thermo Scientific
| Reagents, Solvents and Accessories
39
Thermo Scientific
Hydrolysis Reagents
Constant Boiling (6N) Hydrochloric Acid
For total protein hydrolysis.
Thermo Scientific Hydrochloric Acid is purified and
packaged to ensure a ninhydrin negative blank on hydrolysis.
Convenient, pre-scored ampule packaging of the ready-to-
use HCl maintains reagent integrity. This virtually
eliminates exposure to laboratory atmospheres, fingerprints
and other contaminants resulting from pipetting from bulk
bottles.
Eveleigh and Winter give an excellent description of the
total protein hydrolysis technique using Constant Boiling
Hydrochloric Acid.
1
Standard protein hydrolysis conditions
are 105-110°C for 16-24 hours. At 150°C, this reagent can
hydrolyze peptides in 6 hours.
Highlights:
• Hydrolyzes peptides in 6 hours at 150°C
• Specially purified to give ninhydrin-negative blank on
hydrolysis
• Packaged in ampules to eliminate contamination and
ensure product integrity
References
1. Eveleigh, J.W. and Winter, G.D. (1970). Protein Sequence Determination, Ed
Needleman, S.B., Springer-Verlag, pp 92-95.
2. Blankenship,
et al.
(1989). High-sensitivity amino acid analysis by derivatization
with
o
-Phthaldialdehyde and 9-Fluorescence detection: applications in protein
structure determination.
Anal. Biochem.
178,
227-232.
3. HurIey, J.B.,
et al.
(1984). Isolation and characterization of a cDNA clone for
the subunit of bovine retinal transducin.
Proc. Natl. Acad. Sci. U.S.A.
81,
6948-6952.
4. Lee, K.,
et al.
(1979). Derivatization of cysteine and cystine for fluorescence
amino acid analysis with the
o
-Phthaldialdehyde/2-mercaptoethanol reagent.
J. Biol. Chem.
July 25,
6248-6251.
Ordering Information
Product # Description
Pkg. Size
TS-24308
Hydrochloric Acid
10 x 1 ml ampules
[Constant boiling, Hydrochloric
Acid 6N Sequencing Grade]
Amino Acid Standard H
Our high-purity amino acid calibration standard for protein
hydrolyzates.
The high-purity amino acids of Thermo Scientific Amino Acid
Standard H are ideal for calibrating amino acid analyzers.
To permit standardization of microbiological and other assays,
we have used the L-form configuration. The molar
concentration of each standard is verified by conventional
amino acid analysis methods.
With the exception of cystine, each amino acid is supplied
at a concentration of 2.5 µmoles/ml in 0.1 N HCI. The
following amino acids are included with our Amino Acid
Standard H:
• L-Alanine
L-Leucine
• Ammonia [(NH
4
)
2
SO
4
] L-Lysine•HCl
• L-Arginine
L-Methionine
• L-Aspartic Acid
L-Phenylalanine
• L-Cystine
L-Proline
• L-Glutamic Acid
L-Serine
• Glycine
L-Threonine
• L-Histidine
L-Tyrosine
• L-lsoleucine
L-Valine
Instructions for Use
Thaw Standard H and shake well. Dilute appropriately with
suitable buffers to a concentration compatible with the
full-scale sensitivity of your amino acid analyzer.
Storage
When kept frozen, an unopened vial has an indefinite
storage life. Once the seal is broken, the reagent has a
maximum storage life of six months. Store Amino Acid
Standard H frozen between uses.
Ordering Information
Product # Description
Pkg. Size
TS-20088
Amino Acid Standard H
10 x 1 ml ampules
