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For more information, or to download product instructions,
Introduction to
HPLC Ion Pair Reagents
Introduction to HPLC Ion Pair Reagents
High-purity reagents with the selectivity needed for good
separation.
In the past, reverse-phase HPLC analysis of highly charged
acidic and basic compounds was frustrating and resulted
in poor resolution. Important biomolecules such as amino
acids, peptides, organic acids, polyamines and
catecholamines had to be separated by ion exchange or
by suppression techniques.
Thermo Scientific Ion Pair Reagents enable you to quickly
and efficiently analyze charged compounds using
reverse-phase techniques. Our ion pair reagents are
simply dissolved in the HPLC solvent system, resulting in
the formation of stable chromatographic complexes that
can be separated using reverse-phase columns. By using
the correct ion pair reagents, you achieve:
• Increased or decreased retention, permitting controlled
selectivity
• Resolution of complex ionic mixtures without using ion
exchange columns
• Improved peak symmetry
Reverse-phase ion pair chromatography theories
Two principal theories have been proposed to explain
reverse-phase ion pair chromatography. In the first theory,
small polar ion pair reagents react with the ionized solute,
forming neutral ion pairs. The second theorizes that an
active ion exchange surface is produced in which long
chain, nonpolar anions and cations are absorbed by the
hydrophobic stationary phase.
To optimize chromatographic separations in ion pair elution
systems, high-purity reagents of exceptional optical
transparency are needed. Ion Pair Reagents are specially
purified for ion pair chromatography and provide the
selectivity needed for good separations.
References
1. Bennett, H.P.S.,
et al.
(1981).
Biochemistry.
20,
4530.
2. Starratt, A.N. and Stevens, M.E. (1980).
J. Chromatogr.
194,
421.
3. Burgess, A.W.,
et al.
(1982).
Proc. Natl. Acad. Sci. U.S.A.
79,
5753.
4. Hearn, M.T.W. and Grego, B. (1983).
J. Chromatogr.
255,
125.
5. Shoneshofer, M. and Fenner, A. (1981).
J. Chromatogr.
224,
472.
6. Fischli, W.,
et al.
(1982).
Proc. Natl. Acad. Sci. U.S.A.
79,
5435.
7. Hancock, W.S.,
et al.
(1979).
J. Chromatogr.
168,
377.
8. Hearn, M.T.W.,
et al.
(1978).
J. Chromatogr.
157,
337.
9. Hearn, M.T.W. and Grego, B. (1983).
J. Chromatogr.
255,
125.
10. Hearn, M.T.W. and Grego, B. (1983).
J. Chromatogr.
266,
75.
11. Rivier, J. (1978).
J. Liq. Chrom.
1,
343.
Heptafluorobutyric Acid
An ion pair reagent for the reverse-phase HPLC separation
of proteins and peptides.
Highlights:
• Clear, colorless liquid
• Typical purity is 99.7% by GC; less than 0.1% water
• Sequencing reagent for classical and automated Edman
degradation of peptides and proteins
• Density: 1.645; B.P.: 120°C
• Packaged under nitrogen in amber glass ampules or
bottles
References
1. Hearn, M.T.W. and Hancock, W.S. (1979).
Trends Biochem. Sci.
4,
N58-N62.
2. Bennett, H.P.J.,
et al.
(1980).
J. Liquid Chromatogr.
3,
1353-1366.
3. Bennett, H.P.,
et al.
(1981).
Biochemistry
20,
4530-4538.
Ordering Information
Product # Description
Pkg. Size
✖
TS-25003
Heptafluorobutyric Acid,
100 ml
Sequencing Grade
TS-53104
Heptafluorobutyric Acid,
10 x 1 ml
HPLC Grade
✖
Additional hazardous handling charge.
