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Pesticides in Water
Using 1g 6mL HyperSep C18 Extraction Column
(Part Number: 60108-301)
Sample Preparation
None
Condition C18 Extraction Column
10mL of hexane/acetone (50:50)
10mL of methanol
10mL of DI H
2
O
Apply Sample
Load 1 liter of sample at a rate of 8 to 10mL/minute
Wash Column
20mL of DI H
2
O
Dry Column
Use maximum vacuum pressure for 15 to 30 minutes
Elute Chlorophenoxy Acid Herbicides
10mL of hexane/acetone (50:50)
Concentration/Evaporation
Add 500µL of a keeper solvent (methanol, DMF, other)
Evaporate to 500µL under a nitrogen stream at room
temperature
Pesticides Extracted
α
-hexachlorocychexane
4,4'-DDE
Lindane
Dieldrin
β
-hexachlorocychexane
Endrin
Heptachlor
4,4'-DDD
δ
-hexachlorocychexane
Endosulfan II
Aldrin
4,4'-DDT
Heptachlor
Endrin aldehyde
Endosulfan 1
Enrin sulfate
Environmental
Applications
GC/MS Determination of Phenols in Drinking Water
Using 500mg 6mL HyperSep Retain PEP Extraction Column
(Part Number: 60108-732)
Sample Preparation
Collect 1L of H
2
O
Adjust pH to 2 with 6N HCl
Condition HyperSep Retain PEP Extraction Column
1 x 3mL CH
3
Cl
1 x 3mL CH
3
OH
1 x 3mL 0.05N HCl
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1L of water sample at 20mL/minute
Dry column for 10 to 15 minutes
Wash Column
1 x 10mL H
2
O
Dry column (1 minutes at >10"Hg)
Elute
1 x 10mL CH
3
Cl
1 x 3mL CH
3
Cl
Concentrate the extract to 0.9mL in water bath (40°C)
under a gentle stream of nitrogen
Analysis
Adjust final volume to 1.0mL with CH
3
Cl
Analyze the extract with GC/MS
Recommended GC Column
Part Number
TraceGOLD TG-5MS 30m x 0.25m x 0.25µm
26098-1420