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Extraction of Polycyclic Aromatic Hydrocarbons from Fish
Using QuEChERS Methodology
(Part Number: 60105-211 and 60105-206)
Sample Preparation
Add 5.0g of homogenized fish to a 50mL centrifuge
tube 60105-211
Add 10mL of acetonitrile
Mix by shaking
Mix/vortex for 3 minutes
Centrifuge for 3 minutes at 3400rpm
Clean-up
Add 3mL of supernatant to centrifuge tube 60105-206
Shake for 1 minute
Centrifuge for 1 minute at 3400rpm
Filter supernatant through 0.20µm PTFE membrane
filter (F2500-4)
Analysis
Inject: 15µL on to HPLC
Flow rate: 0.8mL/minute
Mobile phase: 50:50
ACN:waterGradient: linear for 15 min, total run 40 min
Compound
Wavelength nm
naphthalene, acenaphthene and fluorene
315/260
Phenanthrene
366/260
anthracene, fluoranthene, pyrene, benz(a)anthracene,
430/260
chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene,
benzo(a)pyrene, dibenz(a,h)anthracene,
benzo(g,h,i)perylene and dibenzo(a,l)pyrene
(indeno(1,2,3-cd)pyrene)
505/290
Recommended HPLC Column
Part Number
Hypersil GREEN PAH 5µm, 150 x 4.0mm
31105-154030
Determination of Polycyclic Aromatic Hydrocarbon in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Add 1 x 20mL of 10% nitric acid into 1L of H
2
O
Condition HyperSep Retain PEP Extraction Column
1 x 5mL IPA
1 x 5mL H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1L of water sample at 20mL/minute
Wash Column
1 x 5mL (H
2
O 300mL+CH
3
OH 700mL+Na2HPO
4
2.1g+KH
2
PO
4
2g)
Dry column (20 minutes at >10"Hg)
Elute
1 x 4mL (IPA 90mL+acetic acid 10mL+toluene
200mL+petroleum ether 1L)
Concentrate sample
Analysis
Inject onto HPLC
Recommended HPLC Column
Part Number
Hypersil Green PAH 3µm, 150 x 4mm
31103-154030
Environmental
Applications