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Polynuclear Aromatic Hydrocarbons in Pond Water
Using 500mg 3mL HyperSep NH
2
Extraction Column
(Part Number: 60108-518)
Sample Preparation
Filter water sample through a 0.45µm filter
Add 2mL of methanol to 200mL of filtered water sample
Mix and degas sample for 2 minutes
Condition NH
2
Extraction Column
15 to 20mL of methylene
chloride/trichlorotrifluoroethylene (TCTFE)
15 to 20mL of TCTFE
Dry for 5 minutes
15 to 20mL of methanol
20mL of DI H
2
O
Apply Sample
Load 200mL of sample at a rate of 8 to 10mL/minute
Wash Column
20mL of DI H
2
O
Dry Column
Use maximum vacuum pressure for 15 to 30 minutes
Elute Polynuclear Aromatic Hydrocarbons
20mL of TCTFE
Concentration/Evaporation
Evaporate to dryness under a nitrogen stream at room
temperature
Injection/Analysis
Reconstitute with 100µL of TCTFE and inject 1 to 2µL
aliquot onto GC
Polynuclear Aromatic Hydrocarbons Extracted
Naphthalene
Chrysene
Fluorene
B(e)pyrene
Acenaphthene
B(b)fluoranthene
Phenanthrene
B(k)fluoranthene
Anthracene
B(a)pyrene
Fluoranthene
D(a,h)anthracene
Pyrene
B(g,hi)perylene
B(a)anthracene
Indeno(1,2,3-cd)pyrene
Praziquantel in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
To 10mL of water/methanol (95:5, v/v) containing
10ppm atrazine, add 20µL of acetic acid
Condition Retain PEP Extraction Column
2mL of methanol
2mL of DI H
2
O
Apply Sample
Load 4mL samples at 1 to 2mL/minute
Wash Column
1mL of methanol/DI H
2
O (5:95, v/v)
Elute Praziquantel
4mL of methanol
Analysis
Mobile phase: ACN/water (40:60, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 214nm
Recommended HPLC Column
Part Number
Hypersil GOLD 5µm, 250 x 4.6mm
25005-254630
Environmental
Applications