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Phenols in Tap Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
To a sample of tap water add formic acid 1% and
0.5 to 1ppm phenols internal standard
Condition Retain PEP Extraction Column
2mL of methanol
2mL of 1% formic acid
Apply Sample
Load 10mL samples at 1 to 2mL/minute
Wash Column
1mL of 1% formic acid
Elute Phenols
2mL of methanol
Dry Eluate and Reconstitute
Evaporate to full dryness under a stream of nitrogen
Reconstitute the sample to 1mL with methanol/1%
formic acid (1:1)
Analysis
Mobile phase: methanol/formic acid (1:1 v/v)
Recommended HPLC Column
Part Number
Hypersil GOLD aQ 5µm, 250 x 4.6mm
25305-254630
Results
Standard
Blank
Standard (n=3)
Average
Std
Relative Std
1
2
3
Phenol
1.472
0.000
1.367
1.541
1.524
1.477
0.096
100.3
4-nitro phenol
1.469
0.000
1.229
1.308
1.430
1.322
0.101
90.0
m-methylphenol
1.374
0.000
1.294
1.540
1.548
1.461
0.144
106.3
2-chlorophenol
0.613
0.000
0.527
0.684
0.641
0.617
0.081
100.6
2,4-dichlorophenol
1.630
0.000
1.305
1.613
1.621
1.513
0.180
90.392.8
2,4,6-trichlorophenol
1.655
0.000
1.365
1.609
1.511
1.495
0.123
95.690.3
Pentachlorophenol
1.470
0.000
1.259
1.487
1.472
1.406
0.128
95.6
Environmental
Applications
Determination of Phenols in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Collect H
2
O
Condition HyperSep Retain PEP Extraction Column
1 x 3mL methyl tertiary butyl ether (10:90, V/V)
1 x 3mL CH
3
OH
1 x 3mL H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1L of water sample at 20mL/minute
Dry column for 10 to 15 minutes
Wash Column
1 x 10mL DI H
2
O
Dry column (20 minutes at >10"Hg)
Elute
1 x 2mL CH
3
OH
1 x 2mL methyl tertiary butyl ether (10:90 V/V)
Concentrate the collected elute to 1mL with a stream
of nitrogen
Analysis
Inject onto HPLC
Recommended HPLC Column
Part Number
Hypersil GOLD PFP 3µm, 150 x 4.6mm
25403-154630