HyperSep Column Applications

Environmental

Applications

Miticides and Agrochemicals in Honey Bees, Wax and Pollen

Using QuEChERS Methodology

(Part Number: 60105-210, 60105-213, 60105-230 and 60105-308)

Sample Preparation

Wrap sample in aluminum foil

Store on dry ice at -80°C

Beebread and brood – remove from combs store with

beeswax at -20°C until processed

Place 3 grams of sample into a 50mL centrifuge tube

Add 100µL of control solution

Add 1 x 27mL 44% DI water, 55% acetonitrile and

1% glacial acetic acid

Add 1 x 100µL of internal standard

For beebread, reduce particle size by use of a high speed

disperser for 1 minute

For comb wax melt the sample at 80°C in a water bath

followed by cooling to room temperature

Add the contents of 60105-210

Shake

Centrifuge for 1 minute

Clean-Up

Transfer 1mL of supernatant to 60105-230

microcentrifuge tube

Mix/vortex 1 minute

Transfer supernatant to an autosampler vial for LC analysis

Analysis

LC analysis required for neonicotinoids, polar pesticides

and their metabolites

Prepare a dual layer solid-phase extraction cartridge

60105-308 by adding about 80mg of anhydrous

magnesium sulfate to the top frit

Add 1 x 4.0mL of acetone/toluene (7:3 v:v)

Elute solvent to waste under vacuum (60104-230)

Add 1 x 2mL of supernatant to the top of the cartridge

Elute cartridge using 3 to 4mL of acetone/toluene (7:3, v:v)

Evaporator sample at 50°C

Dry eluate to 0.4mL

Inject into GC

Recommended HPLC Column

Part Number

Hypersil GOLD 3µm, 150 x 2.1mm

25003-152130

Nicosulfuron in Solution

Using 60mg 3mL HyperSep Retain-AX Extraction Column

(Part Number: 60107-403)

Sample Preparation

Prepare a 10ppm solution of nicosulfuron in DI H

Condition Retain-AX Extraction Column

1mL of methanol

1mL of DI H

Apply Sample

Load 2mL samples

Wash Column

1mL of 2% ammonia hydroxide

1mL of methanol

Elute Nicosulfuron

2mL of methanol with 2% acetic acid

Analysis

Mobile phase: methanol:2% acetic acid (60:40, v/v)

Flow: 1mL/minute

Temperature: 30°C

Detection: UV 355nm

Recommended HPLC Column

Part Number

Hypersil GOLD aQ 5µm, 250 x 4.6mm

25305-254630