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EPA Method 535 – Analysis of Chloroacetanilide and
Acetamide Herbicide Degradates in Water
Using 500mg 6mL HyperSep Hypercarb Extraction Column
(Part Number: 60106-402)
Sample Preparation
250mL water collected
Condition HyperSep Hypercarb Extraction Column
1 x 20mL ammonium acetate/CH
3
OH
1 x 30mL H
2
O
1 x 3mL H
2
O to top of cartridge
Apply Sample
Load sample at 10 to 15mL/minute
Wash Column
1 x 5mL H
2
O
Dry column
Elute
Insert fresh collection tubes into manifold
1 x 15mL of ammonium acetate/CH
3
OH
Collect eluate at 5mL/minute
Evaporation
Evaporate eluates under a gentle stream of nitrogen in a
heated water bath 60 to 70°C
Analysis
To sample add 1mL 5mM ammonium acetate/CH
3
OH
Inject onto LC/MS/MS
Compound
MRM Transition
Propachlor OA
206/134
Flufenacet OA
224/152
Propachlor ESA
256/80
Flufenacet ESA
274/80
Dimethenamid OA
270/198
Dimethenamid ESA
320/80
Alachlor OA
264/160
Acetochlor OA
264/146
Alachlor ESA
314/80
Metolachlor OA
278/206
Acetochlor ESA
314/80
Metolachlor ESA
328/80
Dimethachlor ESA (sur)
300/80
Butachlor ESA (IS)
356/80
Recommended HPLC Column
Part Number
Hypersil GOLD 3µm, 150 x 2.1mm
25003-152130
Environmental
Applications