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Atrazine in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
To 10mL of water containing 10ppm atrazine, add
20µL of acetic acid
Condition Retain PEP Extraction Column
2mL of methanol
2mL of DI H
2
O
Apply Sample
Load 4mL samples at 1 to 2mL/minute
Wash Column
1mL of DI H
2
O
Elute Atrazine
5mL of methanol
Dry Eluate and Reconstitute
Evaporate to full dryness under a stream of nitrogen
Reconstitute the sample to 0.5mL with water
Analysis
Mobile phase: ACN/0.2% acetic acid (10:90, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 214nm
Recommended HPLC Column
Part Number
Hypersil GOLD 5µm, 250 x 4.6mm
25005-254630
Bentazone in Water
Using 500mg 6mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-206)
Sample Preparation
Adjust the pH of 500mL of water to
≤
3 using
0.5 M H
2
SO
4
Condition Retain PEP Extraction Column
3mL of tetrahydrofuran
5mL of methanol
5mL of DI H
2
O
Apply Sample
Load the 500mL sample at a rate no greater than
5mL/min
Wash Column
5mL of DI H
2
O
Dry column (20 minutes at >10"Hg/full flow for positive
pressure manifold)
Elute Bentazone
Use 0.8mL of methanol to replace the residual water in
the PEP packing
Discard the water which passes through the SPE column
Wait for 2 minutes to make sure the methanol infiltrates
the packing material
Elute the column with 3mL of tetrahydrofuran
Collect the eluate and reconstitute to 3mL using
mobile phase
Environmental
Applications
Bentazone in Water
Alternative Derivatization
Using 500mg 6mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-206)
Sample Preparation
Adjust pH to 3 with H
2
SO
4
Condition HyperSep Retain PEP Extraction Column
1 x 5mL THF
1 x 5mL CH
3
OH
1 x 5mL H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1 x 500mL H
2
O
Elute at 5mL/minute
Wash Column
1 x 5mL H
2
O
Dry column (20 minutes under N
2
)
Elute
1 x 3mL THF
Concentrate sample to 3mL under N
2
Analysis
Inject onto HPLC