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48
4'4-Methylenedianaline in Serum
Using 100mg 1mL HyperSep C18 Extraction Column
(Part Number: 60108-302)
Prepare Sample
None
Condition Verify-CX Extraction Column
3mL of methanol
3mL of DI H
2
O
Apply Sample
Load sample at 1mL/minute
Wash Column
1mL of DI H
2
O
Elute 4'4-Methylenedianaline
0.25mL of methanol containing 1 M ammonium hydroxide
Analysis
Inject 10µL onto HPLC system
Extracted 100 µg/mL sample
Retention time: 3.162 seconds
Mobile phase: methanol/water (50:50)
Flow rate: 1.2mL/min
Injection volume: 10µL
Wavelength: UV 254nm
Recommended HPLC Column
Part Number
Hypersil GOLD 5µm, 250 x 4.6mm
25005-254630
Environmental
Applications
Metal Extraction by SPE
Using HyperSep Aminopropyl, HyperSep Retain-AX, HyperSep SAX
and HyperSep Verify-AX Extraction Columns
Sample Preparation
Prepare sample as appropriate
Condition appropriate HyperSep Extraction Column
NOTE:
Column selection depends on volume of sample,
concentration of metal to be extracted
For a 1mL column
1 x 3mL CH
3
OH
1 x 3mL H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 10 to 50mL of sample water at 1 to 3mL/minute
Wash Column
1 x 10mL H
2
O
Dry column (1 minutes at >10"Hg)
Elute
Acid
Prepare 100mM nitric acid solution
1 x 3mL of nitric acid (100mM ) solution to the column
Flow through at 1 to 3mL/minute
Dilute eluant with H
2
O for analysis
Base
1 x 3mL of triethylamine to the column
Flow through at 1 to 3mL/minute
Dilute eluant with H
2
O for analysis
Analysis
Prepare calibration curves for use with atomic absorption
(AA) or Inductively
Coupled Plasma Atomic Emission Spectroscopy (ICP-AES)
using appropriate metals standards