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Nitrobenzene in Water
Alternative Derivatization
Using 500mg 6mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-206)
Sample Preparation
Add CH
3
OH to water sample to provide a 0.5% solution
Adjust pH to 7
Condition HyperSep Retain PEP Extraction Column
1 x 3mL n-hexane
1 x 5mL CH
3
OH
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1 x 10mL H
2
O
Elute at 5mL/minute
Wash Column
1 x 10mL H
2
O
Dry column (20 minutes at >10"Hg)
Elute
1 x 10mL n-hexane/acetone (90:10, V/V)
Concentrate sample to 1mL
Analysis
Inject onto HPLC
Recommended HPLC Column
Part Number
Hypersil GOLD 3µm, 150 x 4.6mm
25003-154630
Organochlorine Pesticides and PCB Extraction
Using 10g 75mL HyperSep C18 Extraction Column
(Part Number: 60108-703)
Sample Preparation
Adjust pH of sample to 2 using sulfuric acid
Add 1 x 5mL CH
3
OH
Condition HyperSep C18 Extraction Column
1 x 10mL methylene Chloride
1 x 10mL acetone
1 x 10 CH
3
OH
1 x 20mL DI H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load sample at 1 to 3mL/minute
Wash Column
1 x 5mL acetone
Dry column (1 minutes at >10"Hg)
Elute
3 x 10mL CH
3
Cl
Dry the extract by passing it through anhydrous
sodium sulfate
Rinse the collection device with CH
3
Cl
Add the solvent to the sodium sulfate
Analysis
Concentrate the extract
Analyze as appropriate via GC/MS
Recommended GC Column
Part Number
TraceGOLD TG-OCP I 30m x 0.25m x 0.25µm
26078-1420
Clean-Up of Organochlorine Pesticides and PCB Extracts
Using 1g 6mL HyperSep Florisil Column
(Part Number: 60108-431)
Sample Preparation
Add hexane to sample
Condition HyperSep Florisil Extraction Column
1 x 9mL 90:10 hexane/acetone
Apply Sample
Load 2mL of sample at 1 to 2mL/minute
Elute
1 x 9mL of 90:10 hexane/acetone
Collect eluate at 1 to 2mL/minute
Evaporate
Evaporate eluates to 1mL under a gentle stream of
nitrogen <40°C
Reconstitute
Reconstitute sample to a final volume of 2mL with hexane
Environmental
Applications