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Ethametsulfuron in Solution
Using 60mg 3mL HyperSep Retain-AX Extraction Column
(Part Number: 60107-403)
Sample Preparation
Prepare a 10ppm solution of nicosulfuron diluted in 2%
ammonia aqueous solution
Condition Retain-AX Extraction Column
1mL of methanol
1mL of DI H
2
O
Apply Sample
Load 2mL samples
Wash Column
1mL of 2% ammonia hydroxide
1mL of methanol
Elute Ethametsulfuron
2mL of methanol with 2% acetic acid
Analysis
Mobile phase: methanol:2% acetic acid (60:40, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 355nm
Recommended HPLC Column
Part Number
Hypersil GOLD aQ 5µm, 250 x 4.6mm
25305-254630
Glyphosate and Glufosinate by Solid-Phase Anion Exchange Extraction
Using 1g 6mL HyperSep Verify-AX Extraction Column
(Part Number: 60108-732)
Sample Preparation
1L H
2
O adjusted to pH 6
Condition HyperSep Verify-AX Extraction Column
1 x 5mL CH
3
OH
1 x 10mL DI H
2
O
Apply Sample
Load sample at 1 to 3mL/minute
Wash Column
1 x 10mL DI H
2
O
Dry column (10 minutes at >10"Hg)
Elute
1 x 4mL of 1 mol/L HCl/CH
3
OH (4/1)
Add eluant draw through at 1mL/minute
Evaporate
Evaporate under a gentle stream of nitrogen in a water
bath heated to 50°C
Analysis
Add 50µL of MTBSTFA* and 50µL of dimethylformamide
for derivatization
Sonicate for 2 minutes
Heat to 80°C for 30 minutes
Cool to room temperature
Inject onto GC/MS
* Part number TS-48920
Recommended GC Column
Part Number
TraceGOLD TG-17MS 30m x 0.25m x 0.25µm
26089-1420
Environmental
Applications