43 / 144
43
Chlorophenoxy Acid Herbicides in Water
Using 1g 6mL HyperSep C18 Extraction Column
(Part Number: 60108-301)
Sample Preparation
Adjust pH of 1L of water sample to pH 1.0 with
hydrochloric acid
Condition C18 Extraction Column
10mL of hexane/acetone (50:50)
10mL of acidified methanol (5% HCl in methanol)
10mL of DI H
2
O
Apply Sample
Load 1 liter of sample at a rate of 8 to 10mL/minute
Wash Column
10mL of DI H
2
O adjusted to pH 1.0 with HCl
Dry Column
Use maximum vacuum pressure for 15 to 30 minutes
Elute Chlorophenoxy Acid Herbicides
10mL of hexane/acetone (50:50)
Concentrate/Evaporate
Add 500µL of a keeper solvent (methanol, DMF, other)
Evaporate to 500µL under a nitrogen stream at room
temperature
Injection/Analysis
Reconstitute with 100µL of TCTEF and inject at
1 to 2µL onto GC column
Chlorophenoxy Acid Herbicides Extracted
2,4-D acid
2,4,5-trichloro phenoxy propionic acid (Silvex)
Dicamba
Dinitro-sec-butyl phenol
Environmental
Applications
Chlorophenol in Water
Using 500mg 6mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-206)
Sample Preparation
Collect 500mL of H
2
O
Adjust pH to 2 with H
2
SO
4
Condition HyperSep Retain PEP Extraction Column
1 x 5mL CH
3
OH
1 x 5mL H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1 x 500mL H
2
O
Elute at 5mL/minute
Wash Column
1 x 5mL H
2
O
1 x 1mL CH
3
OH
Elute
1 x 3mL THF
Concentrate sample to 3mL under N
2
Analysis
Inject onto HPLC
Recommended HPLC Column
Part Number
Hypersil GOLD PFP 3µm, 150 x 4.6mm
25403-154630
2,4-Dichlorophenol in Water
Using 500mg 6mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-206)
Sample Preparation
Adjust pH to 2 with H
2
SO
4
Condition HyperSep Retain PEP Extraction Column
1 x 5mL CH
3
OH
1 x 5mL H
2
O
NOTE:
Do not allow the cartridge to dry out
Apply Sample
Load 1 x 500mL H
2
O
Elute at 5mL/minute
Wash Column
1 x 5mL H
2
O
1 x 1mL CH
3
OH
Dry column (20 minutes under N
2
)
Elute
1 x 3mL THF
Concentrate sample to 3mL under N
2
Analysis
Inject onto HPLC
Recommended HPLC Column
Part Number
Hypersil GOLD PFP 3µm, 150 x 4.6mm
25403-154630