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Aniline and N,N-Dimethylaniline in Solution
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Prepare solutions of aniline and N,N-dimethylaniline in
DI H
2
O at concentrations of 200, 20 and 2ppm
Condition Retain PEP Extraction Column
3mL of methanol
3mL of DI H
2
O
Apply Sample
Load 3mL samples at 1 to 2mL/minute
Wash Column
1mL of DI H
2
O
Elute Aniline and N,N-Dimethylaniline
3mL of methanol/water (95:5, v/v)
Dry Eluate and Reconstitute
Evaporate to full dryness at room temperature under a
stream of nitrogen
Reconstitute the sample to 1mL with methanol
Analysis
Mobile phase: methanol/water (50:50, v/v)
Recommended HPLC Column
Part Number
Hypersil GOLD aQ 5µm, 250 x 4.6mm
25305-254630
Results
Sample Aniline
Concentration (ppm)
Peak area (av) std
Peak area (av) Retain PEP
Recovery (%) Retain PEP
a
200
360251
213975
60
b
20
28374.536686
20672
56
c
2
3563
2870
81
Sample N,N-dimethylaniline Concentration (ppm)
Peak area (av) std
Peak area (av) Retain PEP
Recovery (%) Retain PEP
a
200
1418380
1398427
98
b
20
174334
159897
92
c
2
19483
18386
94
Acephate in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Prepare a 10mL, 10ppm sample of acephate in ammonium
sulphate aqueous solution (w/w 20%)
Condition Retain PEP Extraction Column
2mL of methanol
2mL of DI H
2
O
Apply Sample
Load 4mL samples at 1 to 2mL/minute
Wash Column
1mL of DI H
2
O
Elute Acephate
5mL of methanol
Dry Eluate and Reconstitute
Evaporate to full dryness under a stream of nitrogen
Reconstitute the sample to 0.5mL with water
Analysis
Mobile phase: ACN/water (40:60, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 214nm
Recommended HPLC Column
Part Number
Hypersil GOLD 5µm, 250 x 4.6mm
25005-254630
Environmental
Applications