57 / 144
57
Thiourea Herbicides in Soil
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Homogenize a soil sample containing thiourea herbicides
in phosphorate buffer solution acid (pH 2.5)
Condition Retain PEP Extraction Column
5mL of methanol
5mL of phosphorate buffer (pH 2.5)
Apply Sample
Load 10mL samples at 1 to 2mL/minute
Wash Column
3mL of phosphorate buffer (pH 2.5)
Elute Thiourea Herbicides
5mL of ACN/phosphorate buffer (pH 7.8) (9:1, v/v)
Dry Eluate and Reconstitute
Evaporate to full dryness under a stream of nitrogen
Reconstitute the sample to 1mL with methanol
Analysis
Mobile phase: ACN-methanol-water (0.2% acetic acid)
Flow: 1mL/minute
Temperature: 30°C
Detection: mass spectrometry
Results
Compound
Recovery (%) Retain PEP
Nicosulfuron
91
Thifensulfuron-methyl
89
Metsulfuron-methyl
89
Sulfometuron-methyl
86
Chlorsulfuron
99
Ethametsulfuron-methyl
81
Tribenuron
15
Bensulfuron-methyl
82
Pyrazosulfuron-ethyl
93
Chlorimuron-ethyl
107
Recommended HPLC Column
Part Number
Hypersil GOLD 5µm, 250 x 4.6mm
25005-254630
Environmental
Applications