3
Thermo Scienti c Poster Note
•
PN ASMS13_Th544_JCole_E 07/13S
ing of more than 500
etermine the value of a
ion by QuEChERS
0 pesticides in matrix.
levels acceptable by EU
es could be determined to
the diversity of compounds
y control. Furthermore,
akes it attractive to use
ectrometry has emerged as
ides due to its high
e of the chemical diversity
l, or even sufficient for a
th LC and GC sample
spectrometer for the
Drug Administration
range of pesticides,
lection of an appropriate
odified QuEChERS
, which is a sample
1
. The QuEChERS extracts
iculture. For the
5 mL of acetonitrile were
CE™ 1310 Gas
s Spectrometer. A
s used on the TRACE
njector was utilized so that
tical column at as low a
was ramped, volatilizing
ed. A slow ramp of 5 ºC/min
d 280 ºC, which is the
d, to achieve optimal
. Figure 1 shows the total
Figure 2 lists the GC
FIGURE 2. Gas Chromatograph Parameters.
LC/MS Instrument Methodol
U-HPLC Method Conditions
Chromatographic analysis was
1250 UHPLC system. The auto
Analytics, Zwingen, Switzerland
Hypersil
™
GOLD aQ column (1
4 is the total ion chromatogram.
FIGURE 5. HPLC Paramete
FIGURE 3. GC-Mass Spectrometer Parameters
GC-Triple Quadrupole Method Conditions
Transitions for all pesticides were taken from the Thermo Scientific™ TSQ 8000
Pesticide Analyzer. These transitions were originally developed with the use of
AutoSRM software, which provided automated SRM development with collision
energies optimized to
±
1 eV. Thermo Scientific TraceFinder™ software was used for
acquisition and processing of the extracted samples. Selecting the appropriate
compounds from the pesticide analyzer automatically populated the SRM acquisition
list in the instrument method and the compound processing parameters in the
Thermo Scientific™ TraceFinder™ software processing method. One ion per
compound was used for quantitation and two additional ions were used for ion ratio
confirmation. Figure 3 lists additional MS parameters used.
FIGURE 4. LC/MS Total Ion C
The analytical column used was a Thermo Scientific™ TraceGOLD™ TG-5SILMS,
with dimensions 30 m x 0.25 mm x 0.25 µm. The liner employed was a baffled,
Siltek™ deactivated inlet liner.
