AN20558_E 07/12S
Application Note 20558
thermoscientific.com/chromatography
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Results
The derivatized glucose produced two peaks,
corresponding to the two cyclic forms of glucose existing
as anomers (Figure 1) and fructose gave rise to one peak.
Good baseline separation between fructose and the two
anomers of derivatized glucose was observed (Figure 2)
using a 14% cyanophenyl polysiloxane phase column.
The stability of the sugars is improved as the acylation
reagent protects the unstable groups, aiding separation on
the chromatographic column.
Conclusion
MBTFA is an ideal derivatization reagent for increasing
the volatility of sugars. This enabled enhanced separation
and detection of fructose and glucose anomers using a
TRACE TR-1701 GC column.
References
Thermo Scientific reagents, solvents and accessories
brochure (Ref: BR20535_E06/12). Available upon
request. ACD labs software to draw chemical structures.
Millivolts
Minutes
0
1000
2000
14
16
18
1
2
2
Figure 1: Acylation of glucose with MBTFA and pyridine
Peak Number
Sugar
t
R
(min)
1
Fructose
16.0
2
Glucose anomers
16.1 and 17.0
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O O
CH
2
OH
CF
3
CF
3
N
CH
3
OH
OH
OH
OH
O
CH
2
OTFA
OTFA
OTFA
O
OTFA
(MBTFA)
Pyridine
OTFA
Figure 2: Chromatogram of the separation of derivatized sugars
on a TR-1701 column.
