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AB52298_E 05/12S
Application Brief 52298
Step 2: Product Ion Study
Now that you have selected your precursor masses, it is
time to find your product masses. AutoSRM will signal
the TSQ 8000 system to acquire product ion scans of your
precursor masses at three collision energies. You are not
required to set up any methods, sequences or data layouts
to accomplish this. AutoSRM automatically takes care of
it. Along with your chromatographic peak and your product
ion spectra, you are presented with a table of the most
intense product ion masses from which to choose. Again,
if you choose, you can have AutoSRM pick for you.
From here you can export your transition list to your
instrument method, or you can send your selected
transitions to an SRM optimization study for further
optimization.
Step 3: SRM Optimization Study
The final step in SRM development is the SRM optimiza-
tion step. Now that you have selected your precursor and
product masses, AutoSRM will acquire those transitions
at multiple collision energies. Because of the fast scanning
capabilities of the TSQ 8000 GC-MS system, three
transitions per compound, each at 10 unique collision
energies, can be analyzed in a single injection. This will
give you a well defined maximum for your collision
energy, as shown in Figure 2 below.
Once complete, AutoSRM allows the simple creation of
the TSQ 8000 instrument method to be ready for routine
analysis.
Figure 2. Collision Energy optimization curve for the
m/z
180 > 109 transition of 1, 2, 3-Tricholobenzene, showing an optimum collision
energy of 25 eV. At this point in the process, the optimized SRM transitions can be exported to your instrument method.
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