Starting from Scratch or from a SIM Method
AutoSRM assists and automates much of the process of
creating SRM transitions from the beginning. If you
already have a SIM method as a starting point, the process
is even faster. Figure 2 shows a collision energy
optimization curve for 1, 2-trichlorobenzene obtained
using AutoSRM. From here, simply export your optimized
transition list to the instrument method, and you are
ready to run. For more details on how AutoSRM assists in
SRM development see
Application Brief AB52298:
Introducing AutoSRM.
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AB52297_E 05/12S
Application Brief 52297
Figure 4. Building a method using TraceFinder software. Simply
select the compounds to acquire and both acquisition list and
processing methods are created for you.
Making Retention Time Adjustments
To make minor retention time adjustments, run a standard
and you are ready to run samples. Retention time
adjustments that are made in data review are pushed back
to your timed acquisition method, simplifying method
maintenance. For more details on how the TSQ 8000
instrument method can be synced to TraceFinder software,
see
Application Brief AB52300: TSQ 8000 GC-MS/MS
Method Sync.
Figure 3. Timed-SRM acquisition list as seen in the TSQ 8000
instrument method. If you already know your SRM transitions,
you can import the list using common industry method formats
or from a spreadsheet. The compound name is listed on the left
side of the bar. The white section in the middle of the bar indicates
the expected peak width. The left portion of the bar indicates
the acquisition time before peak elution, and the right portion
indicates the acquisition time after peak elution. Flexible settings
for tailing peaks are possible.
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Scientific,Austin,TX
USA is ISO Certified.
Figure 2. SRM collision energy optimization curve obtained using
AutoSRM for 1, 2, 3 tricholobenzene.
Starting from an SRM Method
If you already have an SRM method, you can import your
transition list into a TSQ 8000 instrument method either
from a supported instrument method type or from a
spreadsheet with your transition list. The transition list
can then be exported to TraceFinder
™
software for
processing setup or to AutoSRM for further optimization.
Figure 3 shows a timed acquisition list in the TSQ 8000
instrument method. For details on the instrument method,
see
Application Brief AB52299: The TSQ 8000 Triple
Quadrupole GC-MS/MS Instrument Method
.
Starting from Compound Data Store
If you already have a database of SRMs in TraceFinder
software, simply choose your compound list, as shown in
Figure 4. That’s it! TraceFinder software will create your
acquisition list and processing method from your selection.
