AutoSRM
TSQ 8000
Inst. Method
TSQ 8000
Inst. Method
AutoSRM
Method Sync SRM Optimization
Your Starting Point
Unknown SRM Transitions
• Start from the beginning
• Start with existing SIM Method
Known SRM Transitions
• From an existing method
• From SRM Transition list
Optimized SRM Transitions
• From Compound Data Store
• From existing TSQ 8000
GC-MS/MS method
TraceFinder
Software
Thermo Scientific TSQ 8000 GC-MS/MS
Software Overview
Application Brief 52297
Introduction
The advantages of selected reaction monitoring (SRM)
and multiple reaction monitoring (MRM) analysis are
often accompanied by some challenges, especially with
respect to the adoption in the laboratory. These
challenges can be due to the complexity of the mass
spectrometer setup and optimization. Therefore,
laboratories wanting to realize the benefits of MS/MS
must overcome some barriers in the tuning, setup and
optimization to reduce the lead time to get into a
routine workflow.
The Thermo Scientific TSQ 8000 GC-MS/MS was built
with simplicity as a priority. Whether you are managing
retention times, starting with a completely new analysis,
transferring a method from a single quadrupole GC-MS
to a triple quadrupole GC-MS, or porting a known MRM
method from another instrument, the TSQ
™
8000
GC-MS/MS system, through its integrated software tools,
ensures the fastest route to high performance results
routinely.
The TSQ 8000 GC-MS/MS facilitates your lab day:
• maintains actual compound retention times
• imports the last found retention time - for example,
from QC samples - into data acquisition and/or data
processing software
• updates shifting retention times with matrix samples
• compensates for aging columns
• provides an easy update of the retention time after
column clipping
• locks retention time automatically for all compounds in
the acquisition list
• provides safe compound identification based on
retention time or retention index
Software Components
Figure 1 shows a workflow diagram of the three software
components of the TSQ 8000 GC-MS/MS system:
•
AutoSRM Capability:
Purpose-built software for SRM creation
and optimization
•
TSQ 8000 GC-MS/MS Instrument Method:
Offers true timed SRM operation, allowing for high
sensitivity and ease of use for the most complex
SRM methods
•
Thermo Scientific TraceFinder Software:
A multi-platform user-friendly chromatography
analysis software
The sections that follow describe how these components
interact to quickly get you from your starting point to the
routine analysis of your samples.
Figure 1. Workflow diagram of TSQ 8000 GC-MS/MS software.
The design goal of the software was to make it flexible and easy
to use, regardless of what your starting point is in developing your
SRM method.
