Thermo Scientific TSQ 8000 Triple
Quadrupole GC-MS/MS Instrument Method
Application Brief 52299
Introduction
Integration of development, acquisition and processing
methods is a key aspect to a truly productive workflow. As
shown in the flowchart in Figure 1, the Thermo Scientific
TSQ 8000 GC MS/MS instrument method is designed to
be fully integrated with two additional primary software
applications: AutoSRM and Thermo Scientific TraceFinder
software. This enables the convenient migration of
compound information from method development to
method setup to batch acquisition. The TSQ
™
8000
GC-MS/MS instrument method offers true timed-SRM or
timed-SIM acquisition, maximizing instrument sensitivity
through instrument efficiency. With additional ease of use
and performance features, such as full scan/SRM and
scans across the peak checking, performance does not
have to be sacrificed for easy operation.
Flexibility and Ease-of-Use
There are many features that make the TSQ 8000
GC-MS/MS instrument method flexible and easy-to-use,
such as rich import/export capabilities, true retention
time-based acquisition, and scan rate settings that ensure
peak sampling rates are sufficient.
Import/Export Functions
The TSQ 8000 GC-MS/MS instrument method allows
you to import acquisition lists from common method file
types and spreadsheets. Import and export functions are
supported with AutoSRM features and TraceFinder
™
software. You are not required to enter your acquisition
list twice, if you even have to enter it at all, which
provides significant time savings.
Figure 1. The TSQ 8000 instrument method is fully integrated with
AutoSRM and TraceFinder software as part of a complete SRM
development and method management workflow.
Retention Time-Based Acquisition
For SIM, SRM, full scan/SIM, or full scan/SRM, the TSQ
8000 GC-MS/MS instrument method allows acquisition
both in timed acquisition mode or general acquisition
mode. For complex SIM or SRM methods, timed
acquisition offers several advantages. One of these
advantages is the simplification of method management.
For targeted SRM screening applications, it is not
uncommon to have 200 or more transitions in a single
method. Trying to place such a large number of transitions
into discrete segments invariably leads to a handful of
transitions that will be very close to a segment end.
