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Figure 1: MRM chromatograms for (a) Herbal liqueur containing estragole, pulegone & methyl eugenol; (b) Pesto sauce containing estragole and methyl
eugenol; (c) Herbal tea containing thujone, menthofuran, estragole, pulegone, methyl eugenol and coumarin; (d) mixture of seven flavoring standards and
two internal standards
Recoveries (%)
Analyte
Level 1
Level 2
Liquid Matrix
Semi-solid Matrix
Solid Matrix
mg/kg
mg/kg
Level 1
Level 2
Level 1
Level 2
Level 1
Level 2
Thujone
0.1
1.0
95
99
83
121
146
131
Menthofuran
0.1
1.0
121
83
50
83
126
124
Estragole
0.1
1.0
115
90
129
125
123
117
Pulegone
0.1
1.0
107
88
98
105
119
127
Methyl eugenol
0.1
1.0
99
91
106
102
124
113
Coumarin
1.0
10.0
96
97
96
111
107
111
β
-Asarone
0.1
1.0
85
121
62
90
115
116
Table 6: Recoveries (%) for spiked samples at 2 levels
Liquid Matrix
Semi-solid Matrix
Solid Matrix
Analyte
LOD
LOD
LOD
LOQ
LOQ
LOQ
(mg/kg)
(mg/kg)
(mg/kg)
(mg/kg)
(mg/kg)
(mg/kg)
Thujone
0.001
0.005
0.01
0.05
0.003
0.01
Menthofuran
0.001
0.005
0.003
0.01
0.005
0.01
Estragole
0.005
0.01
0.001
0.005
0.005
0.01
Pulegone
0.001
0.005
0.003
0.01
0.001
0.005
Methyleugenol
0.001
0.005
0.01
0.05
0.01
0.05
Coumarin
0.1
0.5
0.1
0.5
0.1
0.5
β
-Asarone
0.001
0.005
0.01
0.05
0.003
0.01
Table 5: Limits of detection and quantification (LODs and LOQs)
