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Application Brief 52300
Thermo Fisher
Scientific,Austin,TX
USA is ISO Certified.
Automatic Retention Time Update
Since you now have a retention time-based instrument
method to acquire your transitions, and a TraceFinder
software method to process the data, how do you
accommodate the clipping of your column and all your
retention times change? If you sync the TraceFinder
method with the acquisition method, all you need to do is
run a standard and update your retention times in Data
Review (Figure 4). Most of these updated retention times
will be determined automatically through automatic peak
detection. The next time you run the method, the
acquisition list will be updated with the new retention
times, eliminating most of the manual work previously
needed to maintain a complex SRM instrument method.
For these complex methods, this can lead to a major time
savings in your daily work.
Compound-Based Scanning
Having the ability to sync the instrument method and the
processing method also allows for easy creation of subsets
of acquisition lists from the CDS. For instance, if you have
a large multi-residue method available, but you are only
interested in the organo-chlorine pesticides instead of the
full set for a particular analysis, simply select the category
for organo-chlorines when creating a processing method
from the CDS. This will not only create a processing
method with your selected compounds, but it will also
create the corresponding acquisition list, limiting the list
to just those compounds. This limited transition list will
increase the dwell time of the selected transitions, and
thus further increase the sensitivity of the TSQ 8000
GC-MS/MS system.
Figure 3. SRM, SIM and full scan rates based on peak width and
scans across the peak
A
B
Figure 4. With Method Sync, when you adjust retention times through
automatic processing in Data Review, retention times will also be
updated in your timed acquisition method. You can update the
retention times one at a time while you QC your data in Data Review
(a), or adjust retention times en masse from an acquired sample (b).
AB52300_E 06/12S
