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Page 5 of 8

Imipramine

Y = 0.00477544*X R^2 = 0.9896 W: Equal

0

200

400

600

800

1000

0

1

2

3

4

5

pg (on-column)

Area Ratio

C6

C7

C5

C4

C3

C2

C1

1000

500

250

125

62.5

31.3

15.6

3.65

Calibration

level

Amount on

column (pg)

% CV

4.00

5.32

7.34

2.28

1.25

4.25

CH3

CH3

N

N

Figure 4: Calibration curve for imipramine in horse urine

0

200

400

600

800

1000

0.0

0.5

1.0

1.5

C3

4.73

C6

4.02

C7

11.24

C5

14.30

C4

14.96

CH3

N

NH

Desipramine

Y = 0.00164932*X R^2 = 0.9835 W: Equal

pg (on-column)

Area Ratio

Calibration

level

Amount on

column (pg)

% CV

500

250

125

1000

62.5

Figure 5: Calibration curve for desipramine in horse urine