321 / 658
:TR
00.01 - 08.5
:MS
G5
6
8
01
)nim( emiT
05
001
05
001
05
001
05
001
05
001
05
001
05
001
05
001
721093 :AA
508413 :AA
194171 :AA
546503 :AA
150575 :AA
8880541 :AA
863505 :AA
7783762 :AA
:TR
00.5 - 00.1
:MS
G5
1
2
3 4
5
)nim( emiT
05
001
05
001
05
001
05
001
05
001
RelativeAbundance
05
001
05
001
05
001
037322 :AA
257943 :AA
741897 :AA
793185 :AA
3793491 :AA
712353 :AA
498357 :AA
038843 :AA
%8.71 = oitaR
A
FF
:TR
03.6 - 03.2
:MS
G5
3 4
5
6
)nim( emiT
05
001
05
001
05
001
05
001
05
001
05
001
05
001
05
001
7817962 :AA
686646 :AA
478677 :AA
445526 :AA
436805 :AA
912407 :AA
018875 :AA
919357 :AA
%5.39 = oitaR
:TR
08.7 - 08.3
:MS
G5
4 5
6
7
)nim( emiT
05
001
05
001
05
001
05
001
05
001
05
001
05
001
05
001
5587661 :AA
829235 :AA
5469572 :AA
8025252 :AA
655549 :AA
906194 :AA
340759 :AA
460161 :AA
%0.87 = oitaR
%8.26 = oitaR
%3.27 = oitaR
%1.27 = oitaR
%8.18 = oitaR
%4.56 = oitaR
%1.501 = oitaR
%9.77 = oitaR
%6.03 = oitaR
%8.93 = oitaR
%4.23 = oitaR
%6.65 = oitaR
%5.83 = oitaR
%9.23 = oitaR
%2.75 = oitaR
%5.67 = oitaR
%6.52 = oitaR
%9.75 = oitaR
%4.11 = oitaR
%1.42 = oitaR
%5.19 = oitaR
%1.24 = oitaR
%5.97 = oitaR
%7.9 = oitaR
%0.33 = oitaR
%0.46 = oitaR
%5.41 = oitaR
%2.13 = oitaR
%0.201 = oitaR
%7.61 = oitaR
I
B
C
D
E
F
G
H
J
K
L
M
N
Q
O
P
R
S
T
Y
U
V
W
X
Z
BB
EE
CC
DD
AA
Figure 2: Qualifier SRM transitions for the 2.5 ng/mL standard. For the compound designators and the target ion ratio %, see Table 1.
enihproM
X/1 :W 7799.0 = 2^R X*2983840.0+67763500.0- = Y
0 02 04 06 08 001
021
041
061
081
002
022
042
062
Lm/gn
1
2
3
4
5
6
7
8
9
01
11
21
31
41
Area Ratio
eniacoC
2^X*178076000.0-X*555817.0+9747640.0- = Y
X/1 :W 6899.0 = 2^R
0 02 04 06 08 001
021
041
061
081
002
022
042
062
Lm/gn
01
02
03
04
05
06
07
08
09
001
011
021
031
041
051
Area Ratio
AMDM
X/1 :W 3899.0 = 2^R X*459011.0+4262010.0 = Y
0 02 04 06 08 001
021
041
061
081
002
022
042
062
Lm/gn
2
4
6
8
01
21
41
61
81
02
22
42
62
82
03
Area Ratio
enimatehpmahteM
X/1 :W 8399.0 = 2^R X*9757050.0+4776310.0- = Y
0 02 04 06 08 001
021
041
061
081
002
022
042
062
Lm/gn
1
2
3
4
5
6
7
8
9
01
11
21
31
41
51
Area Ratio
eniedoC
X/1 :W 0699.0 = 2^R X*1757140.0+81434200.0 = Y
0 02 04 06 08 001
021
041
061
081
002
022
042
062
082
Lm/gn
1
2
3
4
5
6
7
8
9
01
11
21
Area Ratio
eninogcelyozneB
X/1 :W 4999.0 = 2^R X*8285640.0+8836000.0 = Y
0 02 04 06 08 001
021
041
061
081
002
022
042
062
Lm/gn
1
2
3
4
5
6
7
8
9
01
11
21
31
Area Ratio
Figure 3: Calibration curves for select drugs of abuse. Regression curve fitting used 1/x weighting from five replicate injections, where R
2
> 0.993 for all standards.