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Quantitation of Fentanyl and Norfentanyl from
Urine Using On-line High Throughput System
Francois A. Espourteille, Ph. D., Thermo Fisher Scientific, Franklin, MA
Introduction
The use of the Thermo Scientific Aria TLX-4 system with
TurboFlow
™
methods for automated on-line sample
cleanup of a biological sample is well documented in the
literature
1
. The Aria
™
TLX-4 system enhances the
sensitivity, specificity, and precision for mass spectrometric
detection of fentanyl and norfentanyl. Increasing demand
in clinical research laboratories for higher sample
throughput has put the emphasis on automated methods
and platforms that have the ability to quickly ramp up
throughput to meet demand.
The Aria TLX-4 system extracts both fentanyl and its
metabolite, norfentanyl, from the many interferences
found in urine and chromatographically separates them
from each other, before sending them to the mass
spectrometer. TurboFlow extraction methods exclude both
high molecular weight species and salts while the
stationary phase coating retains the analyte(s) through
reverse phase column chemistry. This results in fast,
efficient, on-line separation of fentanyl and its metabolite
prior to introduction into the mass spectrometer.
Goal
• Eliminate the need for SPE
extraction of urine samples for
fentanyl/norfentanyl assay
• Significantly increase sample
throughput by running multiple
samples simultaneously in front
of one mass spectrometer
• Confirm the stability of the on-
line assay
Key Words
• TurboFlow
Technology
• TSQ Quantum
Access
• Clinical Research
• Aria TLX-4
Application
Note: 457
Figure 1: Calibration curves of Fentanyl from 4 channels of Aria TLX-4
System. Data courtesy of Dennis Crouch, Ameritox, LTD.
Figure 2: Excellent Signal/Noise at LOQ for (A) Norfentanyl and (B) Fentanyl at 0.5 ng/mL calibration. Data
courtesy of Dennis Crouch, Ameritox, LTD.
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