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Results and Discussion

The LC-ESI/SRM chromatograms obtained for a blank urine

spiked at 0.5 ng/mL are shown in Figure 2. As presented,

LSD and iso-LSD are separated using the chromatographic

conditions described previously. Identification of LSD is

performed using two characteristic transitions and the

retention time given by its deuterated internal standard.

Linearity

Calibration curves obtained for each compound spiked

in urine samples are presented in Figure 3. Concentration

ranges were comprised between 0.1 ng/mL and 5 ng/mL.

Conclusion

This application note described a sensitive, specific

method developed for the quantitation of lysergide and

metabolites in various biological matrices for forensic use.

For Research Use Only. Not for use in diagnostic procedures.

Quantification Collision

Confirmation Tube lens

Compounds

transition

energy

transition

voltage

LSD

324.0/223.1

30

324.01/207.1

50

Iso-LSD

324.0/223.1

30

324.01/207.1

50

Nor LSD

310.9/208.9

28

310.91/194.0

54

Nor-iso-LSD

310.9/208.9

28

310.91/194.0

54

2-oxo-3-hydroxy-LSD 356.0/236.6

30

356.01/222.0

36

d3-LSD

327.1/210.1

50

327.11/226.2

30

LSD

Nor-LSD

Iso-LSD

2-oxo-3-hydroxy-LSD

R^2 = 0.9998

W: 1/X

R^2 = 0.9989

W: 1/X

R^2 = 0.9987

W: 1/X

R^2 = 0.9996

W: 1/X

12.0

10.0

8.0

5.6

4.8

4.0

3.2

2.4

1.6

0.8

0.0

6.0

4.0

2.0

0.0

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5

0.64

0.56

0.48

0.40

0.32

0.24

0.16

0.00

0.08

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5

0.48

0.40

0.32

0.24

0.16

0.00

0.08

Area Ratio

Area Ratio

ng/mL

ng/mL

Y = 0.00205121+0.11713*X+0.00125718*X^2

Y = -0.0188434+2.1741*X+0.00559891*X^2

Y = 0.00581549+0.0927387*X-0.000858593*X^2

Y = 0.0251564+0.687158*X+0.0721761*X^2

Figure 3: Representative calibration curves from standards spiked in urine