Results and Discussion
The LC-ESI/SRM chromatograms obtained for a blank urine
spiked at 0.5 ng/mL are shown in Figure 2. As presented,
LSD and iso-LSD are separated using the chromatographic
conditions described previously. Identification of LSD is
performed using two characteristic transitions and the
retention time given by its deuterated internal standard.
Linearity
Calibration curves obtained for each compound spiked
in urine samples are presented in Figure 3. Concentration
ranges were comprised between 0.1 ng/mL and 5 ng/mL.
Conclusion
This application note described a sensitive, specific
method developed for the quantitation of lysergide and
metabolites in various biological matrices for forensic use.
For Research Use Only. Not for use in diagnostic procedures.
Quantification Collision
Confirmation Tube lens
Compounds
transition
energy
transition
voltage
LSD
324.0/223.1
30
324.01/207.1
50
Iso-LSD
324.0/223.1
30
324.01/207.1
50
Nor LSD
310.9/208.9
28
310.91/194.0
54
Nor-iso-LSD
310.9/208.9
28
310.91/194.0
54
2-oxo-3-hydroxy-LSD 356.0/236.6
30
356.01/222.0
36
d3-LSD
327.1/210.1
50
327.11/226.2
30
LSD
Nor-LSD
Iso-LSD
2-oxo-3-hydroxy-LSD
R^2 = 0.9998
W: 1/X
R^2 = 0.9989
W: 1/X
R^2 = 0.9987
W: 1/X
R^2 = 0.9996
W: 1/X
12.0
10.0
8.0
5.6
4.8
4.0
3.2
2.4
1.6
0.8
0.0
6.0
4.0
2.0
0.0
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
0.64
0.56
0.48
0.40
0.32
0.24
0.16
0.00
0.08
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
0.48
0.40
0.32
0.24
0.16
0.00
0.08
Area Ratio
Area Ratio
ng/mL
ng/mL
Y = 0.00205121+0.11713*X+0.00125718*X^2
Y = -0.0188434+2.1741*X+0.00559891*X^2
Y = 0.00581549+0.0927387*X-0.000858593*X^2
Y = 0.0251564+0.687158*X+0.0721761*X^2
Figure 3: Representative calibration curves from standards spiked in urine