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Within-day and between-days precisions were de-
termined with QC samples prepared by spiking blank
urine to three concentrations: twice the lowest standard
concentration (QC1), the middle of the calibration range
concentration (QC2), and 80% of the highest standard
concentration (QC3).
Table1: Calibration ranges, within-day precision, and between-days precision
for the lowest QC sample.
Calibration Within-day Between-days
Analyte range (ng/mL) (%RSD) (%RSD)
Citalopram 1-1000 10.7 9.3
Fluoxetine 10-1000 10.4 9.1
Norfluoxetine 10-1000 16.4 11.0
Mirtazapine 1-1000 13.6 12.5
Paroxetine 10-1000 10.2 14.6
Sertraline 10-1000 8.0 15.7
Trazodone 1-1000 11.7 10.6
Venlaflaxine 1-1000 13.6 12.1
Diphenhydramine 1-1000 7.1 7.1
Chlorpheniramine 1-1000 8.2 7.2
Pheniramine 1-1000 7.6 5.6
Cetirizine 5-1000 15.4 15.1
Promethazine 50-1000 4.6 4.2
Nicotine 5-1000 10.7 7.1
Cotinine 5-1000 12.0 8.1
Dextromethorphan 1-1000 8.6 10.9
Topiramate 50-1000 13.2 10.4
Orphenadrine 1-1000 7.2 9.1
Lidocaine 1-1000 11.5 9.4
Phenteramine 10-1000 11.1 13.8
Mesoridazine 5-1000 3.4 4.4
Midazolam 1-1000 14.3 12.2
Chlorpromazine 5-1000 8.0 15.0
Promazine 5-1000 17.1 10.8
Trifluoperazine 5-1000 7.9 17.5
Diltiazem 1-1000 10.1 10.2
Metaprolol 5-1000 10.0 8.5
Verapamil 5-1000 8.7 9.2
Doxylamine 1-1000 14.4 11.7
Hydroxyzine 1-1000 17.9 14.0
Conclusion
An efficient (9 minute), sensitive (LOQ of 1-50 ng/mL),
and precise LC-MS/MS method using TurboFlow tech-
nology was developed for the quantitation of 30 drugs
in human urine. In clinical research, TurboFlow technol-
ogy simplifies sample preparation and improves method
robustness and sensitivity.