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ISO 13485
QMI-SAI Global
Application Note 601
Conclusion
A forensic method for analysis of opiates, opioids, and
their metabolites without hydrolysis has been developed
using the Prelude SPLC system and TSQ Endura MS. By
eliminating the hydrolysis step, the sample preparation
time and analysis cost was drastically reduced. The LC
method on the Prelude SPLC system/TSQ Endura MS
provided ample resolution for all isobaric compounds and
AN64030_E 06/14S
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For Forensic Use Only
an outstanding increase in overall speed of analysis. The
high sensitivity that the TSQ Endura MS provided allowed
for low limits of quantitation of even the least responsive
analytes, like the gluronidated metabolites. The fast SRM
acquisition rate yielded a successful, simultaneous analysis
of 19 compounds with 10 internal standards.
Figure 2. Representative chromatograms for all 19 compounds
0
20
40
60
80
100
Relative Abundance
2.50
2.81
1.86 2.24
Morphine (1.86),
Norcodeine (2.50),
Norhydrocodone (2.81)
0
20
40
60
80
100
Relative Abundance
2.23
2.81
2.50
1.86
Hydromorphone (2.23)
0
20
40
60
80
100
Relative Abundance
2.69
2.45
Codeine (2.45),
Hydrocodone (2.69)
0
20
40
60
80
100
Relative Abundance
2.16
2.42
2.70
Oxymorphone (2.16)
Noroxycodone (2.70)
0
20
40
60
80
100
Relative Abundance
Relative Abundance
Relative Abundance
Relative Abundance
Relative Abundance
Relative Abundance
Relative Abundance
Relative Abundance
Relative Abundance
2.42
2.69
2.15
Dihydrocodeine (2.42)
1.0 1.5 2.0 2.5 3.0 3.5 4.0
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
2.59
1.70
4.14
0.5
1.0
1.5
2.0
2.5
3.0
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
1.65
1.47
1.79
1.65
1.46 1.79
2.38
Oxycodone
6-Acetylcodeine
Hydromorphone 3B
glucuronide (1.65)
6-Acetylmorphine
Normorphine
Codeine 6B glucuronide
Morphine 3B
glucuronide (1.47),
Morphine 6B
glucuronide (1.79)
Oxymorphone 3B
glucuronide
1.46
2.83
1.0 1.5 2.0 2.5 3.0 3.5 4.0
Time (min)