220 / 658
2
Table 2. SRM parameters
Analyte
Precursor
Ion
(Q1)
Product
Ions
(Q3)
CE
(V)
S-lens
(V)
Normorphine
272.0
165.0 59 95
209.0 40 95
Morphine 3b glucuronide
462.1
286.1 52 148
185.2 58 139
Oxymorphone 3b glucuronide
478.1
284.1 47 147
302.1 42 147
Hydromorphone 3b glucuronide
462.1
185.2 58 139
286.1 52 148
Morphine 6b glucuronide
462.1
286.1 52 148
185.2 58 139
Codeine 6b glucuronide
476.2
300.2 31 114
215.2 39 114
6-Acetylmorphine
328.1
165.0 58 112
211.0 39 112
6-Acetylcodeine
342.1
225.1 27 109
165.1 47 109
Dihydromorphine
288.1
185.1 48 95
165.0 59 95
Morphine
286.1
165.1 64 90
185.0 44 119
Oxymorphone
302.0
227.0 40 116
199.1 55 116
Hydromorphone
286.1
185.0 44 119
165.1 64 90
Codeine
300.0
171.0 40 119
199.1 43 119
Dihydrocodeine
302.0
201.1 42 93
199.0 52 93
Norcodeine
286.1
165.1 64 90
181.6 49 90
Oxycodone
316.0
241.1 41 119
256.0 40 119
Noroxycodone
302.1
227.0 41 116
187.0 40 116
Norhydrocodone
286.1
199.0 39 119
241.1 35 119
Hydrocodone
300.0
171.1 40 119
181.1 51 94
Noroxycodone-D
3
305.1
190.1 25 116
Norhydrocodone-D
3
298.1
152.1 62 116
6acetylmorphine-D
6
334.1
165.1 38 116
Morphine 6b glucuronide-D
3
465.1
298.1 32 140
Morphine-D
3
289.1
152.1 61 116
Dihydrocodeine-D
6
308.1
202.1 34 116
Codeine-D
6
306.1
165.1 43 116
Hydromorphone-D
6
292.1
185.1 32 116
Morphine-3b-glucuronide-D
3
465.1
289.1 31 140
Oxycodone-D
6
322.1
218.1 43 116
MS Method Parameters
Instrumentation
TSQ Endura triple quadrupole MS
Ion source
Heated electrospray (HESI II)
Ionization polarity
Positive
Cycle time
0.200 s
Peak width (Q1)
0.7 Da
Peak width (Q3)
0.7 Da
Chrom peak filter width
3.0
Spray voltage
4500 V
Vaporizer temperature
400 °C
Sheath gas pressure
30 (arbitrary units)
Ion sweep gas pressure
1.0 (arbitrary units)
Aux gas pressure
15 (arbitrary units)
Capillary temperature
325 °C
Collision gas pressure
1.5 mTorr
SRM parameters
Refer to Table 2