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Application Note 602
Figure 4 displays the internal standard recovery compared
to that of the calibrators.
Conclusion
• This method gives limits of detection in urine of 50 ng/mL
for EtG and 25 ng/mL for EtS while still maintaining a
wide dynamic range up to 50,000 ng/mL.
• An ion-pairing reagent helps chromatographically
separate the compounds from interferences on the
solvent front, thereby improving limits of detection.
• The TSQ Endura MS is a robust system that provides
accurate results within 5% and good precision all the
way down to the LOQ.
• This method is suitable for forensic toxicology use.
For Forensic Toxicology Use Only.
Figure 4. Internal standard recovery in 23 lots of urine compared to calibrators
0%
20%
40%
60%
80%
100%
120%
140%
160%
Lot
1
Lot
2
Lot
3
Lot
4
Lot
5
Lot
6
Lot
7
Lot
8
Lot
9
Lot
10
Lot
11
Lot
12
Lot
13
Lot
14
Lot
15
Lot
16
Lot
17
Lot
18
Lot
19
Lot
20
Lot
21
Lot
22
Lot
23
%Recovery Compared to Calibrators
EtG-
d
5
EtS-
d
5