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4

Figure 4. Chromatograms showing quantifying and confirming ions with ion ratio at LOQ for each compound in this method.

Codeine LOQ = 2.5 ng/mL

Hydrocodone LOQ = 5 ng/mL

Hydromorphone LOQ = 5 ng/mL

Morphine LOQ = 5 ng/mL

Oxycodone LOQ = 2.5 ng/mL

Oxymorphone LOQ = 2.5 ng/mL