29 / 302
Conclusion
The implementation of Fast-HPLC, coupled with the
online pre-concentration and sample preparation tech-
nique EQuan, yielded analysis of 11 pesticides in drinking
water in less than one-third the time of conventional
HPLC analysis. All of the compounds eluted within three
minutes, which included a one-minute loading time for
the sample to be pre-concentrated on the loading column.
The total run time for the analysis was six minutes. The
Fast-HPLC method can be further shortened to produce
faster chromatographic run times.
The use of large volume injections achieved results
below the MPRL regulatory requirements for each of the
11 pesticides. Because the limits of detection were much
lower than the MPRL values, the integrated peaks yielded
excellent signal-to-noise ratios and allowed for confidence
in reporting the results.
Reference
1
http://www.mhlw.go.jp/index.html(Japanese language version),
http://www.mhlw.go.jp/english/index.html(English language version)
5000
0.5
0.9974
Azoxystrobin
3000
0.5
0.9973
Siduron
300
1
0.9944
Flazasulfuron
4000
0.5
0.9933
Bensulfuron-methyl
200
100
0.9978
Diuron
500
100
0.9345
Carbaryl
50
1
0.9928
Carbofuran
800
5
0.9930
Thiodicarb
800
0.5
0.9972
Tricyclazole
MPRL
(ppt)
Limit of
Detection (ppt)
R
2
Analyte
Table 2: List of calibration curve fit parameters, limits of detection, and
Minimum Performance Reporting Levels (MPRL) for each compound from
the Japanese Ministry of Health, Labour and Welfare. All calibrations were
carried our using a linear curve fit and a weighting factor of 1/X.