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Application Note 572
Conclusion
The use of a divert valve proved suitable for the analysis
of early eluting pesticides in acetonitrile solutions. Good
peak shapes and S/N ratios were achieved and
chromatographic problems, such as peak splitting or
broadening, were overcome. In addition, the injection
volume was increased up to 8 µL, reaching low detection
limits with good linearity and repeatability, even for a
sample concentration of 5 µg/L. It may be possible to
increase the injection volume to 10 µL, and in some cases
up to 15 µL, but with a larger loop volume. After the initial
experiments, we concluded that a 5 µL injection volume is
sufficient to achieve RMS S/N ratio greater than 10.
This technique resolves chromatographic issues involving
interactions of gradient and sample solvent in a simple
way and offers an increased laboratory sample capacity
by avoiding solvent exchange in the final extract.
Reference
1. Jake L. Rafferty, J. Ilja Siepmanna, Mark R. Schure
Journal of Chromatography A
,
2011
,
1218
, 2203–2213.