BR21466-EN Liquid chromatography columns for every biopharma

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A significant amount of information about
a protein can be determined using peptide
mapping. However, additional information
can also be found through characterization
of the whole protein (or light and heavy chain
fragments). Accurate mass and, to some
degree, the heterogeneity of the protein
can be determined. Intact mass is also an
important step after manufacture, to ensure
the stability of the biotherapeutic drug after
storage. Intact mass characterization is
sometimes referred to as “top down” for
the proteins and is challenging compared to
peptide mapping, due to the large mass and
complex nature of the samples.
Intact Mass
Recommended Column
Thermo Scientific ™
2.1 × 50 mm column
Product code: 088648
Thermo Scientific ™
100 µm × 500 mm column
Product code: 164928
• Simplified process for the characterization of
large and complex protein samples
• Reveals information not determined during
peptide mapping, such as accurate mass
and heterogeneity of protein
• Confirms stability of biotherapeutic drug after
manufacturing
Sample Preparation
for increased sensitivity
Sample Handling
The highest level of sample
integrity and sample
information possible
Column
and
UHPLC
Built for every
biopharma workflow
Mass Spectrometry
Thermo Scientific™
Quantify, confirm and characterize
compounds in a single analysis