BR21466-EN Liquid chromatography columns for every biopharma

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Biotherapeutic proteins consist of amino
acids that can be either weakly acid or
weakly basic. Proteins of this size and
complexity, such as monoclonal antibodies,
are far more heterogeneous than small-
molecule drugs. The presence of the
charged state can significantly impact
the structure, stability, binding affinity and
efficacy of the biotherapeutic drug. It is
therefore necessary to understand the profile
of the drug so that charge variants are
identified and removed if necessary. Using
this method, a wide range of antibodies
can be targeted quickly and easily for
determination of charge states.
Charge Variants
Recommended Column
Thermo Scientific ™
4.0 × 250 mm column
Product code: 074625
• Characterization of charge variants, which
can impact structure, stability, binding affinity
and efficacy of biotherapeutics
• Comprehensive determination and
quantification of compounds using sub-5
minute analysis times
• Used in conjunction with Thermo Scientific ™
Sample Preparation
for
increased sensitivity
Sample Handling
The highest level of sample
integrity and sample
information possible
Column UHPLC
Built for every
biopharma workflow
Mass Spectrometry
Thermo Scientific™
Quantify, confirm and characterize
compounds in a single analysis