BR21466-EN Liquid chromatography columns for every biopharma

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Biotherapeutic proteins, like all proteins,
can be digested into a number of smaller
fragments called peptides. Mapping these
peptides via peptide mapping is a commonly
used identity test for proteins. Peptide
mapping is used to verify the identity of
the therapeutic protein and to monitor its
structural integrity and or the presence of
modifications. In conjunction with mass
spectrometry, a full structural survey of the
peptides and their modifications can be
created which will reveal more about the
protein than intact characterization. Peptide
digestion can be made significantly easier
using Thermo Scientific™ SMART Digest™
Kits.
Peptide Mapping
Recommended Column
Thermo Scientific™
2.1 × 250 mm column
Product code: 074812
75 µm × 500 mm column
Product code: 164942
• Comprehensive separation and identification
of peptide fragments
• Confirmation of the structural integrity of the
therapeutic protein through identification of
any modifications
• Reveals more information about the
protein than intact characterization
Sample Preparation
for rapid
and reproducible digestion,
for
increased sensitivity
Sample Handling
The highest level of sample
integrity and sample
information possible
Column UHPLC
Built for every
biopharma workflow
Mass Spectrometry
Quantify, confirm and characterize
compounds in a single analysis