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Conclusion
Endogenous peptides can successfully act as retention time landmarks and
accurately estimate RT in new gradients.
Spectrum libraries capture valuable retention time information.
Our algorithm finds endogenous peptides with consistent elution behavior to
act as standards.
We can accurately predict the retention time of any library peptide by
estimating it relative to the standard peptides. Therefore, comparisons can
more easily be made across datasets with accurate mass and retention time
measurements (AMT). This capability also enables method transfer to
scheduled LC-MRM.
Library-based estimated retention times are closer to the observed times
than predictions made based on hydrophobicity.
References
1. Krokhin OV, Spicer V. (2009) Peptide retention standards and
hydrophobicity indexes in reversed-phase high-performance liquid
chromatography of peptides.
Anal Chem
81(22):9522-30.
Acknowledgments
This research has been funded by the National Cancer Institute (P30
CA076292, P50-CA119997 to EH, R21 CA169979 to EH, R21 CA169980 to JK)
and the American Lung Association (LCD-257857-N to JK) as well as the Moffitt
Foundation.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
For Research Use Only. Not for use in diagnostic procedures.
This information is not intended to encourage use of these products in any manners that might infringe the
intellectual property rights of others.
nt
ptide stored in
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t (Figure 3b).
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icity (Krohkin,
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oo early at the
be due to
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