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Improving Throughput for Highly Multiplexed Targeted Quanti cation Methods Using Novel API-Remote Instrument Control and State-Model
Data Acquisition Schemes
Conclusion
The developments here resulted in the successful qualitative/quantitative analysis for
over 3,000 peptides representing over 2,000 proteins in this complex leukemia cell
digest. Successful quantification was determined for proteins spiked at over a 20-fold
range and the ability to change instrument acquisition parameters for increased
sensitivity.
nge
ide list
in each retention time window
l line and imported into the new
rary information to select unique
nformation used to perform real-time
00 peptides were chosen and 20-fold
:10 could not be calculated using the
in tandem MS/MS scan (panel B, and
FIGURE 6. The benefit of MS/MS scan (with higher S/N) compared to full scan.
Ratio of 1:10 could not be calculated in full scan (panel A), but it could be
calculated in tandem MS/MS scan (panel C).
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Light Channel
Heavy Channel
A
Zoom in
Product ion from heavy
Product ion from light
B
C