482 / 658
Application Note 613
Conclusion
In this study, the utility of direct dilute-and-shoot analysis
of glucuronic acid conjugates of stanozolol by means
of liquid chromatography and high-resolution,
accurate-mass mass spectrometry in sports antidoping
testing was assessed and demonstrated. Characterized and
validated by means of commercially available
3’-OH-stanozolol glucuronide, dilute-and-inject and
confirmation methods were established, which allowed for
the unambiguous identification of stanozolol misuse in
routine doping control samples. Additionally, new
long-term metabolites for the detection of stanozolol
abuse were observed in administration study urine
samples. These new target analytes, assigned as
stanozolol-
N
-glucuronide and 17-epistanozolol-
N
-
glucuronide, were characterized by mass spectrometry,
and hydrolysis experiments. Both proved particularly
useful as target compounds, enabling the determination of
drug abuse for up to 28 days post-administration of 5 mg
of stanozolol. Since high-resolution, accurate-mass mass
spectrometry has been found to be essential for the
successful identification of lowest amounts of stanozolol
metabolites in human urine, expansion of its use in
doping control is encouraged.
Reference
1. Schänzer, W.; Guddat, S.; Thomas, A.; Opfermann, G.;
Geyer, H.; Thevis, M. Expanding analytical possibilities
concerning the detection of stanozolol misuse by means
of high resolution/high accuracy mass spectrometric
detection of stanozolol glucuronides in human sports
drug testing,
Drug Test Anal.
2013
Nov-Dec; 5(11-12),
810-818.
2. Mareck, U.; Geyer, H.; Opfermann, G.; Thevis, M.;
Schänzer, W. Factors Influencing the Steroid Profile in
Doping Control Analysis,
J. Mass Spectrom.
2008
,
43
,
877-891.
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Figure 6. Chromatograms of an authentic doping control routine sample representing an adverse analytical
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