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5
Thermo Scientific Poster Note
•
PN ASMS13_SAMSHA_Prelude_e 06/13S
Conclusion
An LC-MS/MS method for confirmator
NIDA 5 panel using the Prelude SPLC
developed and validated.
The method has LOQs that satisfy the
these 11 drugs.
No matrix interference were observed
The method is simple and fast.
Two-channel multiplexing on Prelude
methods multiplexing in two channels
Acknowledgements
We would like to thank Kent Johnson from P
supplying the comparison samples.
TABLE 2. Intra-method and Inter-method Precision.
for BE, THCA, 6-MAM and
TABLE 3. Recovery of 11 drugs in 6 different urine lots.
For Clinical Research and Forensic Toxicology use only
All trademarks are the property of Thermo Fisher Scien
This information is not intended to encourage use of th
intellectual property rights of others.
Urine Lot
1
2
3
4
5
6
Amphetamine
100
103
98.3
95.8
101
103
MDEA
94.8
99.9
101
98.3
98.5
94.9
MDA
99.6
107
101
100
102
98.7
MDMA
101
100
97.9
99.3
103
102
Methamphetamine 99.8
101
102
96.1
105
98.5
Benzoylecgonine 106
111
97.6
107
109
106
Phencyclidine
88
84.2
81
83.5
85.6
85.9
6-Acetylmorphine 117
109
104
108
104
105
THCA
95.8
90.2
91.2
93.7
97.8
106
Morphine
96.1
99.8
91
90.7
93.9
92.3
Codeine
102
100
102
103
99.7
104
1.1 1.2 1.3 1.4 1.5 1.6
min)
ine
Hydrocodone
Oxycodon
e
on
Precision (RSD%)
Compound
Intra-method
Inter-method
LQC
MQC
HQC
LQC
MQC
HQC
Amphetamine
<15.9 <3.68 <2.86 15.33
3.23
2.32
MDEA
<5.33 <3.46 <5.24
3.65
2.88
3.62
MDA
<6.66 <4.15 <11.89 5.84
2.83
2.52
MDMA
<5.52 <4.34 <3.26
4.68
3.31
3.46
Methamphetamine <5.47 <4.52 <16.63
6.2
4.33
3.79
Benzoylecgonine <2.21 <2.35 <2.53
1.84
1.8
2.2
Phencyclidine
<6.88 <3.56 <4.33
8.8
3.57
3.63
6-Acetylmorphine <5.87 <3.39 <4.11
4.69
3.51
3.67
THCA
<7
<2.8
<2.3
8.3
2.5
3.3
Morphine
<8.2
<10.8
<2.2
8.2
4.8
3
Codeine
<7.35 <5.20 <3.68
5.8
3.99
3.77
method at respective LOQs.
1.2 1.4 1.6 1.8 2.0
(min)
MDEA
MDMA
THCA
0.0.10.20.30.40.50.60.70.80.91.01.1.21.31.4
Time (min)
BE
0.00.20.40.60.81.01.21.41.61.82.0
Time (min)
500 1000 1500 2000 2500
Concentration (ng/mL)
THCA
Y = 1.204e-2X-1.232e-2;R^2: 0.9994;
Origin:Ignore;W:1/X;Area
500 1000 1500 2000 2500
Concentration (ng/mL)
Phencyclidine
Y = 8.447e-3X-1.847e-3;R^2: 0.9916;
Origin:Ignore;W:1/X^2;Area
Concentrations from Prelude SPL
Concentrations from Toxicology Lab (ng/mL)
FIGURE 4. Correlation of data acquired wi
compared with data from a toxicology re
method.