AI-64310-CRFT

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3 Thermo Scientific Poster Note • PN64231-EN 1114S
ast and cost efficient analysis
nalyzed with 8 minute LC-MS
adrupole mass spectrometer.
ethod producing data which
nd robustness.
ounds for analysis. The need
firmation is continually
peed sample analysis time,
ping good data quality.
at 60 C.
spiking solution in methanol.
effects.
d as internal standards.
µm , 5 0x 2.1 mm
cid in water (Fisher
cid in methanol water
TABLE 2. Limits of quantitation a
sample with concentration either
Analytes in this table are listed in
d, including solvent
Results
ass spectrometer with a
s the detector. Two SRM
transition was collected for
e scheme is presented in
performed using Thermo
Analyte
LOQ
(ng/ml)
Pr
%R
Morphine 50
Nicotine 10
Zonisamide 50
Oxymorphone 5
Hydromorphone 20
7-Aminoclonazepam 2
Meprobamate 1
Benzoylecgonine 2
Codeine 20
Pregabalin 100
6-MAM 20
Noroxycodone 20
Oxycodone 10
Norhydrocodone 50
Hydrocodone 20
Amphetamine 5
Carisoprodol 2
Lorazepam 50
a-Hydroxyalprazolam 100
Methamphetamine 1
MDMA 2
Temazepam 10
Clonazepam 50
Nordiazepam 50
%B
0
20
70
100
100
00.0
0
0
Method Performance Evaluation
Calibration standards and QC samples were prepared in artificial urine.
Concentration of the highest calibration standard was limited by concentration of
individual analytes stock solutions (1 mg/mL) purchased from Cerillant.
The following method performance parameters were obtained:
•LOQ: precision within 20% and ion ratio within specified range
•Linearity range: all calibrators accuracy 15%
•Precision: 5 replicates of QC samples
•Matrix effects: Internal standard peak area %Recovery in donor samples (n=40)
calculated against internal standard peak area in water.
Figure 1. SRM transitions acquisition scheme in mass spectrometer method.
Deuterated internal standards for each analyte (not listed at the graph) were
analyzed with the same analyte specific time windows.
Figure 1. Calibration curves and chromatograms of the lowest calibration
standards showing quantifier and qualifier ions for selected analytes.
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
Morphine
nicotine
Zonisamide-neg
Zonisamide
Oxymorphone
Hydromorphone
7-aminoclonazepam
Meprobamatephenobarbital
Benzoylecgonine
gabapentin
codeine
Pregabalin
6 MAM
noroxycodone
butalbital
Oxycodone
norhydrocodone
HydrocodoneAmphetamine
CarisprodolLorazepam
a-hydroxyalprazolam
Methamphetamine
MDMA
ClonazepamTemazepamNordiazepam
AlprazolamNorfentanyltapentadolDiazepamtramadol
Norbuprenorphine
THCA-posTHCA_neg
diphenhydramine
Buprenorphine
Zolpidemdoxepin
Duloxetine
Fentanyl
dextromethorphanCyclobenzaprine
desipraminenortriptylineprotriptylineimipramineamitriptyline
PCP
EDDP
trimipramineclomipramine
Methadone
Retention Time (min)
7-aminoclonazepam
2-1000 ng/mL
Morphine
50-1000 ng/mL
50 ng/mL
7-aminoclonazepam
2-1000 ng/mL
2 ng/mL
Calibra
(ng/m
5
10
20
50
100
200
500
1000
Tramadol
5-1000 ng/mL
Methadone
2-1000 ng/mL
Figure 1. (continued)