AI-64310-CRFT

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5 Thermo Scientific Poster Note • PN64231-EN 1114S
For forensic use o
All trademarks are the proper
This information is not intend
intellectual property rights of
ay precision for the lowest QC
0 ng/mL according to LOQ.
ention time.
Conclusion
 We developed
compounds in
 Method meets
 Matrix effects
standards are
 Short 3.8 minu
throughput on
 Analysis of TH
were evaluate
LOQ was not a
Analyte LOQ
(ng/ml)
Precision
%RSD (n=5)
razolam 2 10.2
rfentanyl 5 14.3
pentadol 2 4.82
zepam 20 5.47
madol 5 5.81
henhydramine 2 3.72
prenorphine 20 9.27
lpidem 1 1.81
xepin 20 10.3
loxetine 20 5.46
ntanyl 1 4.90
xtromethorphan 2 5.69
clobenzaprine 2 5.40
sipramine 50 3.98
rtriptyline 2 6.24
triptyline 50 13.3
ipramine 10 7.28
itriptyline 5 4.63
P 1 2.47
DP 2 3.49
mipramine 10 4.33
mipramine 50 3.66
thadone 2 1.99
Analyte %Rec Analyte %Rec
Morphine-d3 64.3 Alprazolam-d5 83.8
Nicotine-d4 90.2 Norfentanyl-d5 90.7
Zonisamide-13C6 102 Tapentadol-d3 88.7
Oxymorphone-d3 85.7 Diazepam-d5 83.2
Hydromorphone-d6 78.3 Tramadol-d3 89.6
7-Aminoclonazepam-d4 62.9 Diphenhydramine-d3 92.2
Meprobamate-d7 94.5 Buprenorphine-d4 99.1
Benzoylecgonine-d3 78.4 Zolpidem-d6 107
Codeine 92.2 Doxepin-d3 95.2
Pregabalin-d6 105 Duloxetine-d3 82.1
6-MAM-d3 97.3 Fentanyl-d5 104
Noroxycodone-d3 84.4 Dextromethorphan-d3 99.3
Oxycodone-d3 85.4 Cyclobenzaprine-d3 97.4
Norhydrocodone-d3 83.4 Desipramine-d3 97.9
Hydrocodone-d6 83.8 Nortriptyline-d3 101
Amphetamine-d3 72.7 Protriptyline-d3 95.9
Carisoprodol-d7 92.9 Imipramine-d3 99.5
Lorazepam-d4 Not used Amitriptyline-d3 105
a-Hydroxyalprazolam-d5 98.8 PCP-d5 102
Methamphetamine-d5 88.6 EDDP-d3 107
MDMA-d5 89.9 Trimipramine-d3 101
Temazepam-d5 95.0 Clomipramine-d3 98.4
Clonazepam-d4 89.4 Methadone-d3 100
Nordiazepam-d5 87.9
Table 4. Matrix effects: Mean internal standard %Recovery for 50 donor urine
samples.
e
Figure 2. Matrix effects: Internal standard %Recovery in 50 donor samples for
some of the least and the most affected analytes.
0
20
40
60
80
100
120
140
S1 S3 S5 S7 S9 S11S13S15S17S19S21S23S25S27S29S31S33S35S37S39S41S43S45S47S49
6-MAM-d3
OH_Alprazolam-d5
Amitriptyline-d5
Benzoylecgonine-d3
carisoprodol-d7
Codeine-d3
Cyclobenzap-d3
Figure 3. Chromatograms from donor urine samples. Chromatograms selected
are those with lowest analyzed concentration for a given compound.
Fentanyl 64.6 ng/mL Norfentanyl 675 ng/mL
Zolpidem 9.8 ng/mL
Morphine 207 ng/mL
Figure 4. The method e
system: one injection ev
5 ng/mL
2 ng/mL
Figure 3. (continued)