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Quantitation of 47 Forensic Compounds in Urine by HPLC-MS
For forensic use only.
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This information is not intended to encourage use of these products in any manners that might infringe the
intellectual property rights of others.
Conclusion
We developed fast, cost efficient method for quantitative analysis of 47 forensic
compounds in urine.
Method meets laboratory requirements for limit of quantitation.
Matrix effects are observed for some analytes and thus deuterated internal
standards are required.
Short 3.8 minutes acquisition window in 8 minutes method allows to double
throughput on dual channel LC system resulting in analysis of 15 samples per hour.
Analysis of THC-COOH, Norbuprenorphine, Gabapentin, Butalbital, Phenobarbital
were evaluated in this method. Results are not reported because either required
LOQ was not achieved or significant matrix effects were observed.
Analyte
%Rec
m-d5
83.8
yl-d5
90.7
ol-d3
88.7
-d5
83.2
-d3
89.6
dramine-d3
92.2
phine-d4
99.1
-d6
107
3
95.2
e-d3
82.1
d5
104
thorphan-d3
99.3
zaprine-d3
97.4
ine-d3
97.9
ne-d3
101
ne-d3
95.9
e-d3
99.5
ine-d3
105
102
107
ine-d3
101
ine-d3
98.4
e-d3
100
ecovery for 50 donor urine
ery in 50 donor samples for
S39
S41
S43
S45
S47
S49
6-MAM-d3
OH_Alprazolam-d5
Amitriptyline-d5
Benzoylecgonine-d3
carisoprodol-d7
Codeine-d3
Cyclobenzap-d3
s. Chromatograms selected
given compound.
Norfentanyl 675 ng/mL
Zolpidem 9.8 ng/mL
Methadone 5.7 ng/mL
Nicotine 660 ng/mL
Morphine 207 ng/mL
Nortriptyline 106 ng/mL
Benzoylecgonine 81.8 ng/mL
Figure 4. The method executed in multiplexing mode on dual channel Transcend II LC
system: one injection every 4 minutes.
Figure 3. (continued)