360 / 658
QC Sample I (5 injections)
QC Sample II (5 injections)
Calc.
Calc.
Compound Conc (pg) conc. (pg) % Diff % RSD Conc (pg) conc. (pg) % Diff % RSD
EEE
a
200.0
183.2
-8.4
4.6
40.0
37.7
-5.7
5.6
Morphine
200.0
189.2
-5.4
7.6
40.0
40.4
1.0
8.9
Norcodeine
200.0
190.8
-4.6
5.5
40.0
40.1
0.3
7.8
6-Acetylmorphine
200.0
182.2
-8.9
8.1
40.0
41.0
2.6
8.4
Cocaethylene
133.3
120.1
-9.7
7.4
26.7
26.3
-1.5
1.6
Norcocaethylene
200.0
190.6
-4.7
5.5
40.0
42.0
4.9
7.4
Methadol
200.0
184.6
-7.7
9.6
40.0
37.6
-6.1
3.8
EDDP
133.3
121.4
-8.9
4.9
26.7
24.8
-7.1
4.4
Propoxyphene
200.0
190.4
-4.7
4.0
40.0
42.4
6.3
5.8
Methadone
133.3
122.5
-9.5
7.2
26.7
24.9
-6.8
3.9
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www.thermo.comAN62536_E 11/08S
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View additional Thermo Scientific LC/MS application notes at:
www.thermo.com/appnotesY = 92.3738+17.1054*X R^2 = 0.9963 W: 1/X
0
0
Y = 668 9.17+ 133.152*X R^2 = 0.9956 W: 1/X
0
2000
4000
6000
20000
40000
60000
80000
100000
Area
EEE
EEEMS
3
4000
2000
6000
pg on column
pg on column
0
100000
200000
300000
400000
500000
600000
700000
b) Calibration curves using MS/MS and MS
3
product ions
SN: 2
SN: 533
0
10
20
30
40
50
60
70
80
90
MS
3
214.3 196.2
100
MS/MS
214.3
Time (min)
Time (min)
0
10
20
30
40
50
60
70
80
90
100
0.1
0.2
0.3
0.4
0.5
0.6
0.28
0.1
0.2
0.3
0.4
0.5
0.6
0.28
a) Sensitivity and Specificity
RelativeAbundance
RelativeAbundance
%C
2.4
3.7
3.8
9.2
6.9
9.4
11.2
MS
3
3.3
8.6
8.3
8.8
12.0
MS/MS
V
5000
1000
500
100
50
10
5
Amount
(pg on column)
c) %CV for five injections of EEE in oral fluid using MS/MS and MS
3
calibration curves
Figure 4: Analysis of EEE (Ecgonine Ethyl Ester) in oral fluid using MS/MS
and MS
3
spectra product ions
Table 3: Quantification results for the analysis of unknown levels of drugs in oral fluid.
a
based on MS
3
results
Figure 5: Library search results for 6-acetylmorphine using Mass Frontier
software. High match score is highlighted