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Figure 2. Full scan MS chromatogram of a sample containing 12 different analytes
AN63402_E 04/11S
Conclusion
The automated online TurboFlow method with the
LXQ
TM
linear ion trap mass spectrometer allows a fast
and specific approach for the identification of a broad
range of compounds in positive and negative mode in a
single run. The sample preparation time is 15 minutes
with this method as compared to 2 hours with an offline
approach. The LOIs are below 100 ng/mL for more than
90% of the analytes. MS
3
spectra acquisition brings an
additional level of specificity for forensic toxicology
laboratories.
MS
2
spectrum from tramadol and
O-desmethylvenlafaxine
MS
3
spectrum from
O-desmethylvenlafaxine
Tramadol does not give any
MS
3
spectrum
O-desmethylvenlafaxine
Tramadol
Figure 1. Fragmentation of tramadol and O-desmethylvenlafaxine in MS
2
and MS
3
H
3
C CH
3
N
OH
OH
CH
3
O
HO
N
CH
3
H
3
C
Nicotine
Amphetamine
Metamphetamine
Chlorpromazine
Nordiazepam
Diazepam
Perphenazine
Temazepam
Oxazepam
Notryptyline
Benzoylecgonine
Amitryptline
Figure 2. Full scan MS chromatogram of a sample containing 12 different analytes
Figure 1. Fragmentation of tramadol and O-desmethylvenlafaxine in MS
2
and MS
3