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4.0
5.0
6.0
0
10
20
30
40
50
60
70
80
90
100
Relative Abundance
Retention Time (min)
7-Aminonitrazepam
7-Amino-
clonazepam
7-Aminoflunitrazepam
Diazepam
Midazolam
Alprazolam
Nordiazepam
Temazepam
Desalkylflurazepam
2-Hydroxyethylflurazepam
_
-Hydroxyalprazolam
_
-Hydroxytriazolam
Oxazepam
Lorazepam
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Conclusion
We have developed a fast and sensitive LC-MS method
for 14 benzodiazepines in urine using a benchtop Exactive
mass spectrometer with TraceFinder 1.1 software.
TraceFinder 1.1 software is easy to use and effective in
performing quick routine quantitative analysis of benzo-
diazepines in urine. The software enables easy method
development, batch creation, submission and real time
monitoring for clinical research laboratories. The data
review functionality was very useful in quick review
and verification of the calibration accuracy and linearity.
The report templates make selecting and generating reports
with all the necessary information easy and quick.
For Research Use Only. Not for use in diagnostic procedures.
Figure 10. Extracted ion chromatograms of 14 benzodiazepines in urine at their LLOQ (5 ng/mL, mass isolation window=3 ppm)