301 / 658
2.0
2.5
3.0
3.5
4.0
4.5
5.0
Time (min)
7-aminonitrazepam
7-aminoclonazepam
7-aminoflunitrazepam
midazolam
α
-hydroxytriazolam
α
-hydroxyalprazolam
2-hydroxyethylflurazepam
lorazepam
oxazepam
alprazolam
desalkylflurazepam
temazepam
nordiazepam
diazepam
Figure 2
Figure 2. SRM chromatogram of 14 benzodiazepines and metabolites in urine at a concentration of 25 ng/mL
Compound
Lot A
Lot B
Lot C
Lot D
Lot E
Lot F
Lot G
Lot H
2-hydroxyethyl-flurazepam
83.6
94.7
113
106
131
107
101
102
7-amino-clonazepam
90.9
92.4
93.1
90.0
95.5
98.5
92.0
92.2
7-aminoflunitrzepam
97.1
98.0
100
101
97.6
108
94.9
96.5
7-aminonitrazepam
88.9
99.6
94.9
101
94.0
101
96.5
89.3
a
-hydroxyalprazolam
107
104
90.9
112
105
106
113
99.3
a
-hydroxytriazolam
95.5
107
101
96.9
87.5
90.7
109
107
alprazolam
108
101
107
110
107
98.9
92.7
95.5
desalkylflurazepam
108
89.3
104
97.6
103
98.9
105
103
diazepam
105
102
113
106
105
111
89.3
103
lorazepam
104
93.1
94.9
95.8
91.1
94.4
108
107
midazolam
113
111
110
101
104
107
105
95.6
nordiazepam
112
99.3
112
109
98.4
109
95.6
102
oxazepam
96.4
91.5
96.7
96.9
92.0
99.3
95.1
96.0
temazepam
105
98.2
99.1
95.5
101
99.1
98.2
101
Table 1. Percent recovery of 14 benzodiazepines in eight lots of urine
Conclusion
A robust dilute and shoot method with simple and easy
sample preparation for the analysis of 14 benzodiazepines
in 6.5 minutes was developed for forensic toxicology use.
The data window and total run time make this method
amenable to multiplexing with the Thermo Scientific Aria
Transcend system. Multiplexing with the Transcend™
system would result in a run time of 3.5 minutes per
sample.